DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jang, YS | ko |
dc.contributor.author | Jung, YR | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.contributor.author | Mm, JM | ko |
dc.contributor.author | Lee, JW | ko |
dc.contributor.author | Oh, DB | ko |
dc.contributor.author | Kang, HA | ko |
dc.contributor.author | Kwon, O | ko |
dc.contributor.author | Jang, SH | ko |
dc.contributor.author | Song, H | ko |
dc.contributor.author | Lee, SJ | ko |
dc.contributor.author | Kang, KY | ko |
dc.date.accessioned | 2011-01-28T05:27:07Z | - |
dc.date.available | 2011-01-28T05:27:07Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007-09 | - |
dc.identifier.citation | APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.73, pp.5411 - 5420 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.uri | http://hdl.handle.net/10203/21898 | - |
dc.description.abstract | Shuttle vectors carrying the origins of replication that function in Escherichia coli and two capnophilic rumen bacteria, Mannheimia succiniciproducens and Actinobacillus succinogenes, were constructed. These vectors were found to be present at ca. 10 copies per cell. They were found to be stably maintained in rumen bacteria during the serial subcultures in the absence of antibiotic pressure for 216 generations. By optimizing the electroporation condition, the transformation efficiencies of 3.0 X 10(6) and 7.1 X 10(6) transformants/mu g DNA were obtained with M. succiniciproducens and A. succinogenes, respectively. A 1.7-kb minimal replicon was identified that consists of the rep gene, four iterons, A+T-rich regions, and a dnaA box. It was found that the shuttle vector replicates via the theta mode, which was confirmed by sequence analysis and Southern hybridization. These shuttle vectors were found to be suitable as expression vectors as the homologous fumC gene encoding fumarase and the heterologous genes encoding green fluorescence protein and red fluorescence protein could be expressed successfully. Thus, the shuttle vectors developed in this study should be useful for genetic and metabolic engineering of succinic acid-producing rumen bacteria. | - |
dc.description.sponsorship | We thank Stefan Schwarz (Federal Agricultural Research Center, Germany) for kindly providing us with the plasmid pMVSCS1. This work was supported by the Genome-Based Integrated Bioprocess Development Project (no. 2005-01294) of the Ministry of Science and Technology through the Korea Science and Engineering Foundation (KOSEF). Further support by the LG Chem Chair Professorship and the IBM SUR program and by KOSEF through the Center for Ultramicrochemical Process Systems is appreciated. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.title | Construction and characterization of shuttle vectors for succinic acid-producing rumen bacteria | - |
dc.type | Article | - |
dc.identifier.wosid | 000249246700003 | - |
dc.identifier.scopusid | 2-s2.0-34548480682 | - |
dc.type.rims | ART | - |
dc.citation.volume | 73 | - |
dc.citation.beginningpage | 5411 | - |
dc.citation.endingpage | 5420 | - |
dc.citation.publicationname | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | - |
dc.identifier.doi | 10.1128/AEM.01382-07 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Jang, YS | - |
dc.contributor.nonIdAuthor | Jung, YR | - |
dc.contributor.nonIdAuthor | Mm, JM | - |
dc.contributor.nonIdAuthor | Lee, JW | - |
dc.contributor.nonIdAuthor | Oh, DB | - |
dc.contributor.nonIdAuthor | Kang, HA | - |
dc.contributor.nonIdAuthor | Kwon, O | - |
dc.contributor.nonIdAuthor | Jang, SH | - |
dc.contributor.nonIdAuthor | Song, H | - |
dc.contributor.nonIdAuthor | Lee, SJ | - |
dc.contributor.nonIdAuthor | Kang, KY | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | MANNHEIMIA-SUCCINICIPRODUCENS | - |
dc.subject.keywordPlus | ANAEROBIOSPIRILLUM-SUCCINICIPRODUCENS | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | DNA-REPLICATION | - |
dc.subject.keywordPlus | BOVINE RUMEN | - |
dc.subject.keywordPlus | COPY-NUMBER | - |
dc.subject.keywordPlus | PLASMIDS | - |
dc.subject.keywordPlus | SEQUENCE | - |
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