DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, SangYup | ko |
dc.contributor.author | Lee, Y | ko |
dc.date.accessioned | 2011-01-28T05:24:41Z | - |
dc.date.available | 2011-01-28T05:24:41Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2003-06 | - |
dc.identifier.citation | APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.69, pp.3421 - 3426 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.uri | http://hdl.handle.net/10203/21896 | - |
dc.description.abstract | A heterologous metabolism of polyhydroxyalkanoate (PHA) biosynthesis and degradation was established in Escherichia coli by introducing the Ralstonia eutropha PHA biosynthesis operon along with the R. eutropha intracellular PHA depolymerase gene. By with this metabolically engineered E. coli, enantiomerically pure (R)-3-hydroxybutyric acid (R3HB) could be efficiently produced from glucose. By employing a two-plasmid system, developed as the PHA biosynthesis operon on a medium-copy-number plasmid and the PHA depolymerase gene on a high-copy-number plasmid, R3HB could be produced with a yield of 49.5% (85.6% of the maximum theoretical yield) from glucose. By integration of the PHA biosynthesis genes into the chromosome of E. coli and by introducing a plasmid containing the PHA depolymerase gene, R3HB could be produced without plasmid instability in the absence of antibiotics. This strategy can be used for the production of various enantiomerically pure (R)-hydroxycarboxylic acids from renewable resources. | - |
dc.description.sponsorship | This work was supported by the Ministry of Commerce, Industry and Energy, the Brain Korea 21 program of the Ministry of Education, and the National Research Laboratory program of the Ministry of Science and Technology. Further support from the Center for Ultramicrochemical Process Systems is appreciated. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.title | Metabolic engineering of Escherichia coli for production of enantiomerically pure (R)-(-)-hydroxycarboxylic acids | - |
dc.type | Article | - |
dc.identifier.wosid | 000187156200050 | - |
dc.identifier.scopusid | 2-s2.0-0037641053 | - |
dc.type.rims | ART | - |
dc.citation.volume | 69 | - |
dc.citation.beginningpage | 3421 | - |
dc.citation.endingpage | 3426 | - |
dc.citation.publicationname | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Lee, Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | ALCALIGENES-EUTROPHUS H16 | - |
dc.subject.keywordPlus | BACTERIAL POLYHYDROXYALKANOATES | - |
dc.subject.keywordPlus | RALSTONIA-EUTROPHA | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | PHB | - |
dc.subject.keywordPlus | (R)-3-HYDROXYBUTANOATE | - |
dc.subject.keywordPlus | STRAINS | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | GENES | - |
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