Production of poly(3-hydroxybutyrate) by fed-batch culture of recombinant Escherichia coli with a highly concentrated whey solution

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Fermentation strategies for the production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes were developed. The pa-stat fed-batch cultures of E. coli CGSC 4401 harboring pJC4, a stable plasmid containing the ii, latus PHA biosynthesis genes, were carried out with a concentrated whey solution containing 280 g of lactose equivalent per liter. Final cell and PHB concentrations of 119.5 and 96.2 g/liter, respectively, were obtained in 37.5 h, which resulted in PHB productivity; of 2.57 g/liter/h.
Publisher
AMER SOC MICROBIOLOGY
Issue Date
2000-08
Language
English
Article Type
Article
Keywords

BETA-HYDROXYBUTYRIC ACID; BACTERIAL POLYHYDROXYALKANOATES; CLONING; GENES

Citation

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.66, no.8, pp.3624 - 3627

ISSN
0099-2240
URI
http://hdl.handle.net/10203/21892
Appears in Collection
CBE-Journal Papers(저널논문)
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