DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, JI | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2011-01-28T01:06:27Z | - |
dc.date.available | 2011-01-28T01:06:27Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1999-10 | - |
dc.identifier.citation | APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.65, no.10, pp.4363 - 4368 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.uri | http://hdl.handle.net/10203/21881 | - |
dc.description.abstract | Fermentation strategies for production of high concentrations of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] with different 3-hydroxyvalerate (3HV) fractions by recombinant Escherichia coli harboring the Alcaligenes latus polyhydroxyalkanoate biosynthesis genes were developed. Fed-batch cultures of recombinant E. coli with the pa-stat feeding strategy facilitated production of high concentrations and high contents of P(3HB-co-3HV) in a chemically defined medium. When a feeding solution was added in order to increase the glucose and propionic acid concentrations to 20 g/liter and 20 mM, respectively, after each feeding, a cell dry weight of 120.3 g/liter and a relatively low P(3HB-co-3HV) content, 42.5 wt%, were obtained. Accumulation of a high residual concentration of propionic acid in the medium was the reason for the low P(3HB-co-3HV) content. An acetic acid induction strategy was used to stimulate the uptake and utilization of propionic acid. When a fed-batch culture and this strategy were used, we obtained a cell concentration, a P(3HB-co-3HV) concentration, a P(3HB-co-3HV) content, and a 3HV fraction of 141.9 g/liter, 88.1 g/liter, 62.1 wt%, and 15.3 mol%, respectively. When an improved nutrient feeding strategy, acetic acid induction, and oleic acid supplementation were used, we obtained a cell concentration, a P(3HB-co3HV) concentration, a P(3HB-co-3HV) content, and a 3HV fraction of 203.1 g/liter, 158.8 g/liter, 78.2 wt%, and 10.6 mol%, respectively; this resulted in a high level of productivity, 2.88 g of P(3HB-co3HV)/liter-h. | - |
dc.description.sponsorship | This work was supported by the Ministry of Science and Technology and by LG Chemicals, Ltd. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.title | High-level production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by fed-batch culture of recombinant Escherichia coli | - |
dc.type | Article | - |
dc.identifier.wosid | 000082959100011 | - |
dc.identifier.scopusid | 2-s2.0-0039109649 | - |
dc.type.rims | ART | - |
dc.citation.volume | 65 | - |
dc.citation.issue | 10 | - |
dc.citation.beginningpage | 4363 | - |
dc.citation.endingpage | 4368 | - |
dc.citation.publicationname | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Choi, JI | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | BACTERIAL POLYHYDROXYALKANOATES | - |
dc.subject.keywordPlus | POLY-(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE) | - |
dc.subject.keywordPlus | POLY(3-HYDROXYBUTYRATE) | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | GENES | - |
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