DC Field | Value | Language |
---|---|---|
dc.contributor.author | Huh, Yun Suk | ko |
dc.contributor.author | Jeong, Chang-Moon | ko |
dc.contributor.author | Chang, Ho Nam | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.contributor.author | Hong, Won-Hi | ko |
dc.contributor.author | Park, Tae Jung | ko |
dc.date.accessioned | 2011-01-24T05:48:26Z | - |
dc.date.available | 2011-01-24T05:48:26Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2010-03 | - |
dc.identifier.citation | BIOMICROFLUIDICS, v.4, no.1 | - |
dc.identifier.issn | 1932-1058 | - |
dc.identifier.uri | http://hdl.handle.net/10203/21775 | - |
dc.description.abstract | A protein separation technology using the microfluidic device was developed for the more rapid and effective analysis of target protein. This microfluidic separation system was carried out using the aqueous two-phase system (ATPS) and the ionic liquid two-phase system (ILTPS) for purification method of the protein sample, and the three-flow desalting system was used for the removal of salts from the sucrose-rich sample. Partitioning of the protein sample was observed in ATPS or ILTPS with the various pHs. The microdialysis system was applied to remove small molecules, such as sucrose and salts in the microfluidic channel with the different flow rates of buffer phase. A complex purification method, which combines microdialysis and ATPS or ILTPS, was carried out for the effective purification of bacteriorhodopsin (BR) from the purple membrane of Halobacterium salinarium, which was then analyzed by sodium dodecyl sulfatepolyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight. Furthermore, we were able to make a stable three-phase flow controlling the flow rate in the microfluidic channel. Our complex purification methods were successful in purifying and recovering the BR to its required value. | - |
dc.description.sponsorship | This work was supported in part by the IT Leading R&D Support Project from the Ministry of Knowledge Economy through KEIT and WCU World Class University program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology Grant No. R32-2008-000-10142-0. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | AMER INST PHYSICS | - |
dc.title | Rapid separation of bacteriorhodopsin using a laminar-flow extraction system in a microfluidic device | - |
dc.type | Article | - |
dc.identifier.wosid | 000276209300013 | - |
dc.identifier.scopusid | 2-s2.0-77957859668 | - |
dc.type.rims | ART | - |
dc.citation.volume | 4 | - |
dc.citation.issue | 1 | - |
dc.citation.publicationname | BIOMICROFLUIDICS | - |
dc.identifier.doi | 10.1063/1.3298608 | - |
dc.contributor.localauthor | Chang, Ho Nam | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.localauthor | Hong, Won-Hi | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | biological techniques | - |
dc.subject.keywordAuthor | bioMEMS | - |
dc.subject.keywordAuthor | electrophoresis | - |
dc.subject.keywordAuthor | microchannel flow | - |
dc.subject.keywordAuthor | molecular biophysics | - |
dc.subject.keywordAuthor | multiphase flow | - |
dc.subject.keywordAuthor | pH | - |
dc.subject.keywordAuthor | photoionisation | - |
dc.subject.keywordAuthor | photon stimulated desorption | - |
dc.subject.keywordAuthor | proteins | - |
dc.subject.keywordAuthor | purification | - |
dc.subject.keywordAuthor | separation | - |
dc.subject.keywordAuthor | time of flight mass spectra | - |
dc.subject.keywordPlus | HALOBACTERIUM-HALOBIUM | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | PURPLE MEMBRANE | - |
dc.subject.keywordPlus | METAL-IONS | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | RHODOPSIN | - |
dc.subject.keywordPlus | CHIP | - |
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