Rapid and ultrasensitive detection of microRNA by target-assisted isothermal exponential amplification coupled with poly (thymine)-templated fluorescent copper nanoparticles

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We devised a novel method for rapid and ultrasensitive detection of target microRNA (miRNA) by employing target-assisted isothermal exponential amplification (TAIEA) combined with poly (thymine)-templated fluorescent copper nanoparticles (CuNPs) as signaling probes. The target miRNA hybridizes to the unimolecular template DNA and works as a primer for the extension reaction to form double-stranded product, which consequently generates two nicking endonuclease recognition sites. By simultaneous nicking and displacement reactions, exponential amplification generates many poly (thymine) strands as final products, which are employed for the synthesis of fluorescent CuNPs. Based on the fluorescent signal from CuNPs, target miRNA is detected as low as 0.27 fM around 1 h of total analysis time. The diagnostic capability of this system has been successfully demonstrated by reliably detecting target miRNA from different cell lysates, showing its great potential towards real clinical applications
Publisher
IOP PUBLISHING LTD
Issue Date
2016-10
Language
English
Article Type
Article
Keywords

ROLLING CIRCLE AMPLIFICATION; LABEL-FREE DETECTION; CANCER-CELLS; ELECTROCHEMICAL DETECTION; SIGNAL AMPLIFICATION; SENSITIVE DETECTION; BREAST-CANCER; PROTEIN; NANOCLUSTERS; BIOGENESIS

Citation

NANOTECHNOLOGY, v.27, no.42

ISSN
0957-4484
DOI
10.1088/0957-4484/27/42/425502
URI
http://hdl.handle.net/10203/214251
Appears in Collection
CBE-Journal Papers(저널논문)
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