DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이상엽 | ko |
dc.contributor.author | 장호남 | ko |
dc.date.accessioned | 2010-12-14T05:31:06Z | - |
dc.date.available | 2010-12-14T05:31:06Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1995 | - |
dc.identifier.citation | KSBB JOURNAL , v.10, no.2, pp.137 - 142 | - |
dc.identifier.issn | 1225-7117 | - |
dc.identifier.uri | http://hdl.handle.net/10203/21011 | - |
dc.description.abstract | When Escherichia coli W, which is able to utilize sucrose as a carbon source, harboring a high-copy-number plasmid (pSYL105) containing the Alcaligenes eultrophus polyhydroxyalkanoate(PHA) biosynthetic genes was cultured in a defined medium, the final poly(3-hydroxybutyric acid), PHB, concentration obtained was as low as 0.21g/l. Ten different complex nitrogen sources were, therefore, examined for their ability to enhance PHB synthesis when supplemented to a defined medium. Addition of tryptone, casamino acids, casein hydrolysate, or soy bean hydrolysate enhanced PHB synthesis most significantly, resulting in more than 10 times higher PHB concentration compared with that obtained in a defined medium. Furthermore, PHB yield on sucrose was also increased by more than a 10 fold by the addition of these complex nitrogen sources, which suggested that PHB might be efficiently produced by the recombinant E. coli W(pSYL105) using sucrose as a carbon source | - |
dc.description.sponsorship | 본 연구는 한국과학기술원과 한국과학재단에서 지원받았으며 이에 감사드립니다. | en |
dc.language | Korean | - |
dc.language.iso | ko | en |
dc.publisher | 한국생물공학회 | - |
dc.title | Sucrose를 탄소원으로 재조합 대장균에 의한 Poly(3-Hydroxybutyric Acid) 합성시 복합질소원이 미치는 영향 | - |
dc.title.alternative | Effect of Complex Nitrogen Sources on Poly(3-Hydroxybutyric Acid) Synthesis by Recombinant Escherichia coli Using Sucrose as a Carbon Source | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.citation.volume | 10 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 137 | - |
dc.citation.endingpage | 142 | - |
dc.citation.publicationname | KSBB JOURNAL | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | 이상엽 | - |
dc.contributor.localauthor | 장호남 | - |
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