국소 표면 플라즈몬 공명 (LSPR) 기반 비표지 바이오칩 제작 및 바이오센서로의 응용Fabrication of Label-Free Biochips Based on Localized Surface Plasmon Resonance (LSPR) and Its Application to Biosensors
In the past decade, we have observed rapid advances in the development of biochips in many fields including
medical and environmental monitoring. Biochip experiments involve immobilizing a ligand on a solid substrate
surface, and monitoring its interaction with an analyte in a sample solution. Metal nanoparticles can display
extinction bands on their surfaces. These charge density oscillations are simply known as the localized surface
plasmon resonance (LSPR). The high sensitivity of LSPR has been utilized to design biochips for the label-free
detection of biomolecular interactions with various ligands. LSPR-based optical biochips and biosensors are
easy to fabricate, and the apparatus cost for the evaluation of optical characteristics is lower than that for the
conventional surface plasmon resonance apparatus. Furthermore, the operation procedure has become more
convenient as it does not require labeling procedure. In this paper, we review the recent advances in LSPR
research and also describe the LSPR-based optical biosensor constructed with a core-shell dielectric
nanoparticle biochip for its application to label-free biomolecular detections such as antigen-antibody
interaction.