Separation of intracellular proteins from Candida utilis using reverse micelles in a spray column

Abstract

Recovery of intracellular proteins from Candida utilis by reverse micellar extraction in a simple spray column was studied. The effects of varying the number of circulations, the flow rate, and the column length on the amount of extracted proteins were examined. The optimal number of circulations and the flow rate for the extraction of proteins were 2 and 0.2 ml/sec, respectively. The amount of proteins recovered by stripping was greatly affected by the number of circulations, which showed dramatic increase during 7 to 9 circulations.