Nanoscopic observation of a gold nanoparticle-conjugated protein using near-field scanning optical microscopy

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This Study describes a single gold nanoparticle (AuNP)-based observation of biomolecular interaction using a near-field scanning microscope (NSOM) in transmission mode. To observe streptavidin molecules, a glass surface was first patterned with a micro-scale line of (3-aminopropy)trimethoxysilane (APTMS) by micro-contact printing (mu CP) with a Subsequent reaction of N-hydroxysuccinimide (NHS)-biotin. The AuNP-conjugated streptavidin was then applied to the biotin-modified glass Surface and NSOM was employed to detect the resulting specific interiction between streptavidin and biotin oil the glass surface. Using the optical and topological images generated from the NSOM analysis, the interaction Could be observed at the nanoscopic scale. This Study demonstrates that the NSOM is a powerful tool for the detection of protein interactions at the nanoscopic level when the protein is conjugated with AuNPs. (C) 2008 Elsevier B.V. All rights reserved.
Publisher
ELSEVIER SCIENCE BV
Issue Date
2008-09
Language
English
Article Type
Article; Proceedings Paper
Keywords

HNF-1-ALPHA MUTATIONS; METAL NANOPARTICLES; FORCE MICROSCOPY; RECOGNITION; MICROARRAY; DIAGNOSIS; SURFACE; PROBE; DNA

Citation

ULTRAMICROSCOPY, v.108, pp.1115 - 1119

ISSN
0304-3991
URI
http://hdl.handle.net/10203/20933
Appears in Collection
CBE-Journal Papers(저널논문)
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