DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, So Young | ko |
dc.contributor.author | Park, Si Jae | ko |
dc.contributor.author | Kim, Won Jun | ko |
dc.contributor.author | Yang, Jung Eun | ko |
dc.contributor.author | Lee, Hyuk | ko |
dc.contributor.author | Shin, Jihoon | ko |
dc.contributor.author | Lee, Sang Yup | ko |
dc.date.accessioned | 2016-07-04T03:11:40Z | - |
dc.date.available | 2016-07-04T03:11:40Z | - |
dc.date.created | 2016-05-10 | - |
dc.date.created | 2016-05-10 | - |
dc.date.created | 2016-05-10 | - |
dc.date.created | 2016-05-10 | - |
dc.date.issued | 2016-04 | - |
dc.identifier.citation | NATURE BIOTECHNOLOGY, v.34, no.4, pp.435 - + | - |
dc.identifier.issn | 1087-0156 | - |
dc.identifier.uri | http://hdl.handle.net/10203/209023 | - |
dc.description.abstract | Poly(lactate-co-glycolate) (PLGA) is a widely used biodegradable and biocompatible synthetic polymer. Here we report one-step fermentative production of PLGA in engineered Escherichia coli harboring an evolved polyhydroxyalkanoate (PHA) synthase that polymerizes D-lactyl-CoA and glycolyl-CoA into PLGA. Introduction of the Dahms pathway enables production of glycolate from xylose. Deletion of ptsG enables simultaneous utilization of glucose and xylose. An evolved propionyl-CoA transferase converts D-lactate and glycolate to D-lactyl-CoA and glycolyl-CoA, respectively. Deletion of adhE, frdB, pflB and poxB prevents by-product formation. We also demonstrate modulation of the monomer fractions in PLGA by overexpressing ldhA and deleting dld to increase the proportion of D-lactate or by deleting aceB, glcB, glcD, glcE, glcF and glcG to increase the proportion of glycolate. Incorporation of 2-hydroxybutyrate is prevented by deleting ilvA or feeding strains with l-isoleucine. The utility of our approach for generating diverse forms of PLGA is shown by the production of copolymers containing 3-hydroxybutyrate, 4-hydroxybutyrate or 2-hydroxyisovalerate | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | One-step fermentative production of poly(lactate-co-glycolate) from carbohydrates in Escherichia coli | - |
dc.type | Article | - |
dc.identifier.wosid | 000373616100026 | - |
dc.identifier.scopusid | 2-s2.0-84963516758 | - |
dc.type.rims | ART | - |
dc.citation.volume | 34 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 435 | - |
dc.citation.endingpage | + | - |
dc.citation.publicationname | NATURE BIOTECHNOLOGY | - |
dc.identifier.doi | 10.1038/nbt.3485 | - |
dc.contributor.localauthor | Lee, Sang Yup | - |
dc.contributor.nonIdAuthor | Choi, So Young | - |
dc.contributor.nonIdAuthor | Park, Si Jae | - |
dc.contributor.nonIdAuthor | Kim, Won Jun | - |
dc.contributor.nonIdAuthor | Yang, Jung Eun | - |
dc.contributor.nonIdAuthor | Lee, Hyuk | - |
dc.contributor.nonIdAuthor | Shin, Jihoon | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | GLYCOLIC ACID PRODUCTION | - |
dc.subject.keywordPlus | POLYLACTIC ACID | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | POLYESTERS | - |
dc.subject.keywordPlus | POLYHYDROXYALKANOATES | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | COPOLYMERS | - |
dc.subject.keywordPlus | POLYMERS | - |
dc.subject.keywordPlus | SYNTHASE | - |
dc.subject.keywordPlus | PATHWAY | - |
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