DC Field | Value | Language |
---|---|---|
dc.contributor.author | Song, H | ko |
dc.contributor.author | Kim, TY | ko |
dc.contributor.author | Choi, BK | ko |
dc.contributor.author | Choi, SJ | ko |
dc.contributor.author | Nielsen, LK | ko |
dc.contributor.author | Chang, Ho Nam | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2010-12-08T09:09:08Z | - |
dc.date.available | 2010-12-08T09:09:08Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2008-05 | - |
dc.identifier.citation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.79, no.2, pp.263 - 272 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | http://hdl.handle.net/10203/20846 | - |
dc.description.abstract | This study presents a novel methodology for the development of a chemically defined medium (CDM) using genome-scale metabolic network and flux balance analysis. The genome-based in silico analysis identified two amino acids and four vitamins as non-substitutable essential compounds to be supplemented to a minimal medium for the sustainable growth of Mannheimia succiniciproducens, while no substitutable essential compounds were identified. The in silico predictions were verified by cultivating the cells on a CDM containing the six non-substitutable essential compounds, and it was further demonstrated by observing no cell growth on the CDM lacking any one of the non-substitutable essentials. An optimal CDM for the enhancement of cell growth and succinic acid production, as a target product, was formulated with a single-addition technique. The fermentation on the optimal CDM increased the succinic acid productivity by 36%, the final succinic acid concentration by 17%, and the succinic acid yield on glucose by 15% compared to the cultivation using a complex medium. The optimal CDM also lowered the sum of the amounts of by-products (acetic, formic, and lactic acids) by 30%. The strategy reported in this paper should be generally applicable to the development of CDMs for other organisms, whose genome sequences are available. | - |
dc.description.sponsorship | This work was supported by the Genome-based Integrated Bioprocess Project of the Ministry of Science and Technology (No. 2005-01294) through the Korea Science and Engineering Foundation (KOSEF). Further supports by the LG Chem Chair Professorship and by the KOSEF through the Center for Ultramicrochemical Process Systems are appreciated. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | SPRINGER | - |
dc.title | Development of chemically defined medium for Mannheimia succiniciproducens based on its genome sequence | - |
dc.type | Article | - |
dc.identifier.wosid | 000255253200012 | - |
dc.identifier.scopusid | 2-s2.0-42549143731 | - |
dc.type.rims | ART | - |
dc.citation.volume | 79 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 263 | - |
dc.citation.endingpage | 272 | - |
dc.citation.publicationname | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.identifier.doi | 10.1007/s00253-008-1425-2 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Chang, Ho Nam | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Song, H | - |
dc.contributor.nonIdAuthor | Kim, TY | - |
dc.contributor.nonIdAuthor | Choi, BK | - |
dc.contributor.nonIdAuthor | Choi, SJ | - |
dc.contributor.nonIdAuthor | Nielsen, LK | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | chemically defined medium | - |
dc.subject.keywordAuthor | Mannheimia succiniciproducens | - |
dc.subject.keywordAuthor | metabolic network | - |
dc.subject.keywordAuthor | flux balance analysis | - |
dc.subject.keywordAuthor | succinic acid | - |
dc.subject.keywordPlus | SUCCINIC ACID PRODUCTION | - |
dc.subject.keywordPlus | CAPNOPHILIC RUMEN BACTERIUM | - |
dc.subject.keywordPlus | LISTERIA-MONOCYTOGENES | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | MINIMAL MEDIUM | - |
dc.subject.keywordPlus | AMINO-ACIDS | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | FERMENTATION | - |
dc.subject.keywordPlus | METHODOLOGY | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.