3D interference printing enables the single-step production of multilayered ultralong nanochannel arrays with nano-scale regularity. The superior depth-of-focus of this technique realizes a state-of-the-art nanostructure which has intensively stacked 32 layers of inchlong, horizonontal nanochannels with sub-100 nm holes in a monolithic matrix (approximate to 15 mu m). This exceptional structure can be integrated into microfluidic devices, facilitating high-flux rheological platforms using nanocapillarity.