DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hong, Soon Ho | ko |
dc.contributor.author | Lee, Sang Yup | ko |
dc.date.accessioned | 2010-12-06T07:06:38Z | - |
dc.date.available | 2010-12-06T07:06:38Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2004-07 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.9, no.4, pp.252 - 255 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | http://hdl.handle.net/10203/20761 | - |
dc.description.abstract | A pfl IdhA double mutant Escherichia coli strain NZN111 was used to produce succinic acid by overexpressing the E. coli malic enzyme gene (sfcA). This strain, however, produced a large amount of malic acid as well as succinic acid. After the analyses of the metabolic pathways, the fumB gene encoding the anaerobic fumarase of E. coli was co-amplified to solve the problem of malic acid accumulation. A plasmid, pTrcMLFu, was constructed, which contains an artificial operon (sfcA-fumB) under the control of the inducible trc promoter. From the batch culture of recombinant E. coli NZN111 harboring pTrcMLFu, 7 g/L of succinic acid was produced from 20 g/L of glucose, with no accumulation of malic acid. From the metabolic flux analysis the strain was found under reducing power limiting conditions by severe reorientation of metabolic fluxes. | - |
dc.description.sponsorship | We thank David P. Clark for kindly providing the E.coli strain NZN111. This work was financially supported by the Korean Systems Biology Research Program (M10309020000-03B5002-00000) of the Ministry of Science and Technology (MOST). Further supports by the IBM-SUR program, LG Chem Chair Professorship and BK21 program are appreciated. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.title | Enhanced production of succinic acid by metabolically engineered Escherichia coli with amplified activities of malic enzyme and fumarase | - |
dc.type | Article | - |
dc.identifier.wosid | 000223710100003 | - |
dc.identifier.scopusid | 2-s2.0-28344441063 | - |
dc.type.rims | ART | - |
dc.citation.volume | 9 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 252 | - |
dc.citation.endingpage | 255 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.identifier.doi | 10.1007/BF02942339 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, Sang Yup | - |
dc.contributor.nonIdAuthor | Hong, Soon Ho | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | succinic acid | - |
dc.subject.keywordAuthor | malic enzyme | - |
dc.subject.keywordAuthor | fumarase | - |
dc.subject.keywordAuthor | metabolic engineering | - |
dc.subject.keywordPlus | FLUX ANALYSIS | - |
dc.subject.keywordPlus | OVEREXPRESSION | - |
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