DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, SangYup | ko |
dc.contributor.author | Chang, Ho Nam | ko |
dc.date.accessioned | 2010-12-06T05:19:51Z | - |
dc.date.available | 2010-12-06T05:19:51Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1994-07 | - |
dc.identifier.citation | JOURNAL OF ENVIRONMENTAL POLYMER DEGRADATION, v.2, no.3, pp.169 - 176 | - |
dc.identifier.issn | 1064-7546 | - |
dc.identifier.uri | http://hdl.handle.net/10203/20754 | - |
dc.description.abstract | When a recombinant Escherichia coli XL1-Blue harboring pSYL105 was cultured in a complex medium, a poly(3-hydroxybutyric acid) (PHB) concentration of 7.16 g/L was obtained in 48 h. However, a PHB concentration of only 0.91 g/L was obtained in 60 h by culturing in a defined medium. Also, fed-batch culture in a defined medium resulted in considerably lower PHB accumulation than in a complex medium. With the aim to produce a high concentration of PHB at a reduced medium cost, we examined 10 complex nitrogen sources for their ability to promote PHB synthesis in a defined medium. Tryptone, casamino acids, and casein hydrolysate promoted PHB synthesis to a higher extent than the others tested. PHB synthesis was also enhanced during fedbatch cultures when a defined medium was supplemented with various complex nitrogen sources. With tryptone supplementation a PHB concentration of 66.7 g/L could be obtained in 44 h. Yeast extract was less effective for promoting PHB synthesis than tryptone. Corn steep liquor, which did not enhance PHB synthesis significantly, could promote PHB synthesis considerably when supplemented together with yeast extract in both flask and fed-batch cultures. © 1994 Plenum Publishing Corporation. | - |
dc.description.sponsorship | We would like to thank Yoo Kyung Lee for her great technical assistance. We thank Dr. A. Steinbuchel and Dr. K. Gerdes for providing us with pSK2665 and pKG1022, respecively, used in the construction of pSYL105. We also thank Miwon C., DGF Stoess, and Ajinomoto Co. for providing us with corn steep liquor, collagen hydrolysate, and soybean hydrolystae, respectively. This work was supported by the Korea Science and Engineering Foundation. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | Kluwer Academic/Plenum Publishers | - |
dc.title | Effect of complex nitrogen source on the synthesis and accumulation of poly(3-hydroxybutyric acid) by recombinant Escherichia coli in flask and fed-batch cultures | - |
dc.type | Article | - |
dc.identifier.scopusid | 2-s2.0-0028478475 | - |
dc.type.rims | ART | - |
dc.citation.volume | 2 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 169 | - |
dc.citation.endingpage | 176 | - |
dc.citation.publicationname | JOURNAL OF ENVIRONMENTAL POLYMER DEGRADATION | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.localauthor | Chang, Ho Nam | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | complex nitrogen source | - |
dc.subject.keywordAuthor | defined medium | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.subject.keywordAuthor | fed-batch culture | - |
dc.subject.keywordAuthor | poly(3-hydroxybutyric acid) | - |
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