Construction of E.coli-C.acetobutylicum shuttle vectors and transformation of C.acetobutylicum strains

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dc.contributor.authorLee, SangYupko
dc.contributor.authorBENNETT, GNko
dc.contributor.authorPAPOUTSAKIS, ETko
dc.date.accessioned2010-12-06T05:14:11Z-
dc.date.available2010-12-06T05:14:11Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued1992-05-
dc.identifier.citationBIOTECHNOLOGY LETTERS, v.14, no.5, pp.427 - 432-
dc.identifier.issn0141-5492-
dc.identifier.urihttp://hdl.handle.net/10203/20753-
dc.description.abstractSeveral E. coli-C. acetobutylicum shuttle vectors were constructed and used to transform two C. acetobutylicum strains ATCC 824 and NCIMB 8052. Other than pSYL2, none of these vectors were able to transform ATCC 824 due to the presence of a restriction system. However, all of them could transform NCIMB 8052 with efficiencies of 8 x 10(2) - 6 x 10(3) transformants per mu-g DNA.-
dc.description.sponsorshipThis work was carried out at Northwestern University and was supported by Grant #BCS-8912209 from the National Science Foundation, USA.en
dc.languageEnglish-
dc.language.isoen_USen
dc.publisherCHAPMAN HALL LTD-
dc.titleConstruction of E.coli-C.acetobutylicum shuttle vectors and transformation of C.acetobutylicum strains-
dc.typeArticle-
dc.identifier.wosidA1992HX12200016-
dc.identifier.scopusid2-s2.0-0026751475-
dc.type.rimsART-
dc.citation.volume14-
dc.citation.issue5-
dc.citation.beginningpage427-
dc.citation.endingpage432-
dc.citation.publicationnameBIOTECHNOLOGY LETTERS-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.contributor.localauthorLee, SangYup-
dc.contributor.nonIdAuthorBENNETT, GN-
dc.contributor.nonIdAuthorPAPOUTSAKIS, ET-
dc.type.journalArticleArticle-
dc.subject.keywordPlusBACILLUS-SUBTILIS-
dc.subject.keywordPlusPLASMIDS-
dc.subject.keywordPlusSTREPTOCOCCUS-
dc.subject.keywordPlusCLONING-
dc.subject.keywordPlusPERFRINGENS-
dc.subject.keywordPlusRESISTANCE-
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