DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wong, HH | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2010-12-03T07:03:10Z | - |
dc.date.available | 2010-12-03T07:03:10Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998-07 | - |
dc.identifier.citation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.50, no.1, pp.30 - 33 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | http://hdl.handle.net/10203/20691 | - |
dc.description.abstract | Recombinant Escherichia coli strain GCSC 6576, harboring a high-copy-number plasmid containing the Ralstonia eutropha genes for polyhydroxyalkanoate (PHA) synthesis and the E. coli ftsZ gene, was employed to produce poly-(3-hydroxybutyrate) (PHB) from whey, pH-stat fed-batch fermentation, using whey powder as the nutrient feed, produced cellular dry weight and PHB concentrations of 109 g l(-1) and 50 g l(-1) respectively in 47 h. When concentrated whey solution containing 210 g l(-1) lactose was used as the nutrient feed, cellular dry weight and PHB concentrations of 87 g l(-1) and 69 g l(-1) respectively could be obtained in 49 h by pH-stat fed-batch culture. The PHB content was as high as 80% of the cellular dry weight. These results suggest that cost-effective production of PHB is possible by fed-batch culture of recombinant E. coli using concentrated whey solution as a substrate. | - |
dc.description.sponsorship | The PHA biosynthesis genes used in the con- struction of pSYL107 were kindly provided by A. SteinbuÈ chel. We thank Mary Berlyn for kindly providing the strain GCSC 6576. We also thank Anton Middelberg for his valuable suggestions during his visit to the KAIST. This work was supported by The Korea Science and Engineering Foundation. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | SPRINGER | - |
dc.title | Poly(3-hydroxybutyrate) production from whey by high density cultivation of recombinant Escherichia coli | - |
dc.type | Article | - |
dc.identifier.wosid | 000075299500004 | - |
dc.identifier.scopusid | 2-s2.0-0031877849 | - |
dc.type.rims | ART | - |
dc.citation.volume | 50 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 30 | - |
dc.citation.endingpage | 33 | - |
dc.citation.publicationname | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Wong, HH | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | BETA-HYDROXYBUTYRIC ACID | - |
dc.subject.keywordPlus | POLYHYDROXYALKANOATE PRODUCTION | - |
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