DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ahn, WS | ko |
dc.contributor.author | Park, SJ | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2010-12-02T07:03:32Z | - |
dc.date.available | 2010-12-02T07:03:32Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2001-02 | - |
dc.identifier.citation | BIOTECHNOLOGY LETTERS, v.23, no.3, pp.235 - 240 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | http://hdl.handle.net/10203/20652 | - |
dc.description.abstract | A new fermentation strategy using cell recycle membrane system was developed for the efficient production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes. By cell recycle, fed-batch cultivation employing an external membrane module, the working volume of fermentation could be constantly maintained at 2.3 l. The final cell concentration, PHB concentration and PHB content of 194 g l(-1), 168 g l(-1) and 87%, respectively, were obtained in 36.5 h by the pH-stat cell recycle fed-batch culture using whey solution concentrated to contain 280 g lactose l(-1) as a feeding solution, resulting in a high productivity of 4.6 g PHB l(-1) h(-1). | - |
dc.description.sponsorship | We thankMary Berlyn for kindly providing the CGSC strains. This work was supported by the Korea- Australia International Cooperative Project from the Ministry of Science and Technology (MOST). | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | SPRINGER | - |
dc.title | Production of poly(3-hydroxybutyrate) from whey by cell recycle fed-batch culture of recombinant Escherichia coli | - |
dc.type | Article | - |
dc.identifier.wosid | 000166354000014 | - |
dc.identifier.scopusid | 2-s2.0-0035133622 | - |
dc.type.rims | ART | - |
dc.citation.volume | 23 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 235 | - |
dc.citation.endingpage | 240 | - |
dc.citation.publicationname | BIOTECHNOLOGY LETTERS | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Ahn, WS | - |
dc.contributor.nonIdAuthor | Park, SJ | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | cell recycle fed-batch culture | - |
dc.subject.keywordAuthor | poly(3-hydroxybutyrate) | - |
dc.subject.keywordAuthor | recombinant E. coli | - |
dc.subject.keywordAuthor | whey | - |
dc.subject.keywordPlus | BACTERIAL POLYHYDROXYALKANOATES | - |
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