DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jin Y.L. | ko |
dc.contributor.author | Jang Y.-S. | ko |
dc.contributor.author | Lee J. | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.contributor.author | Papoutsakis E.T. | ko |
dc.date.accessioned | 2010-11-30T08:27:50Z | - |
dc.date.available | 2010-11-30T08:27:50Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2009-10 | - |
dc.identifier.citation | BIOTECHNOLOGY JOURNAL, v.4, no.10, pp.1432 - 1440 | - |
dc.identifier.issn | 1542-0752 | - |
dc.identifier.uri | http://hdl.handle.net/10203/20542 | - |
dc.description.abstract | To improve butanol selectivity, Clostridium acetobutylicum M5(pIMP1E1AB) was constructed by adhE1-ctfAB complementation of C. acetobutylicum M5, a derivative strain of C. acetobutylicum ATCC 824, which does not produce solvents due to the lack of megaplasmid pSOL1. The gene products of adhE1-ctfAB catalyze the formation of acetoacetate and ethanol/butanol with acid reassimilation in solventogenesis. Effects of the adhE1-ctfAB complementation of M5 were studied by batch fermentations under various pH and glucose concentrations, and by flux balance analysis using a genome-scale metabolic model for this organism. The metabolically engineered M5(pIMP1E1AB) strain was able to produce 154 mM butanol with 9.9 mM acetone at pH 5.5, resulting in a butanol selectivity (a molar ratio of butanol to total solvents) of 0.84, which is much higher than that (0.57 at pH 5.0 or 0.61 at pH 5.5) of the wild-type strain ATCC 824. Unlike for C. acetobutylicum ATCC 824, a higher level of acetate accumulation was observed during fermentation of the M5 strain complemented with adhE1 and/or ctfAB. A plausible reason for this phenomenon is that the cellular metabolism was shifted towards acetate production to compensate reduced ATP production during the largely growth-associated butanol formation by the M5(pIMP1E1AB) strain. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA. | - |
dc.description.sponsorship | This work was supported by the Korea-Australia Collaborative Research Project from the Ministry of Knowledge Economy (#10030795). We would like to thank Dr. Jin Hwan Park for his leadership in this project. Further supports by GS Caltex and Bio- FuelChem are appreciated. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | Wiley - VCH Verlag GmbH & CO. KGaA | - |
dc.title | Metabolic engineering of Clostridium acetobutylicum M5 for highly selective butanol production | - |
dc.type | Article | - |
dc.identifier.scopusid | 2-s2.0-70449575862 | - |
dc.type.rims | ART | - |
dc.citation.volume | 4 | - |
dc.citation.issue | 10 | - |
dc.citation.beginningpage | 1432 | - |
dc.citation.endingpage | 1440 | - |
dc.citation.publicationname | BIOTECHNOLOGY JOURNAL | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Jin Y.L. | - |
dc.contributor.nonIdAuthor | Jang Y.-S. | - |
dc.contributor.nonIdAuthor | Lee J. | - |
dc.contributor.nonIdAuthor | Papoutsakis E.T. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Aldehyde/alcohol dehydrogenase | - |
dc.subject.keywordAuthor | Butanol | - |
dc.subject.keywordAuthor | Clostridium acetobutylicum | - |
dc.subject.keywordAuthor | Coenzyme A transferase | - |
dc.subject.keywordAuthor | Trade-off curve analysis | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.