Use of a triiodide resin for isolation of axenic cultures of microalgal Nannochloropsis gaditana

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Triiodide resin (TR) was used to generate axenic cultures of microalgae by employing the antibacterial capability of triiodide. A Nannochloropsis gaditana culture contaminated with bacteria was passed through a column filled with TR using the gravity flow. Based on analyses of flow cytometry and vital staining using a fluorescent dye SYTOX Green, three cycles of TR treatments remarkably reduced the number of viable bacteria but had little effects on the microalgae. This novel approach is a simple, rapid, and cost-effective method that can be used to isolate axenic cultures of microalgae.
Publisher
ELSEVIER SCI LTD
Issue Date
2015-09
Language
English
Article Type
Article
Keywords

BACTERIA; ALGAE; WATER; BIOMASS

Citation

BIORESOURCE TECHNOLOGY, v.191, pp.391 - 394

ISSN
0960-8524
DOI
10.1016/j.biortech.2015.03.082
URI
http://hdl.handle.net/10203/203924
Appears in Collection
CBE-Journal Papers(저널논문)
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