DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wong, HH | ko |
dc.contributor.author | Kim, YC | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.contributor.author | Chang, Ho Nam | ko |
dc.date.accessioned | 2010-11-24T08:17:26Z | - |
dc.date.available | 2010-11-24T08:17:26Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998-11 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOENGINEERING, v.60, no.3, pp.271 - 276 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | http://hdl.handle.net/10203/20339 | - |
dc.description.abstract | The effect of post-induction nutrient feeding strategies on the production of bioadhesive protein using an IPTG inducible expression system in Escherichia coil was investigated. Cells were cultured in an exponential fed-batch mode to the OD600 of ca. 100 (48 gDCW/L) prior to induction. Six different post-induction nutrient feeding strategies (pH-stat, exponential, constant and linear change in feeding rate with three different slopes) were then applied, and bioadhesive protein production was examined. It was found that post-induction cell growth was independent of nutrient feeding rate. However, bioadhesive protein production was significantly affected by post-induction feeding strategies. Linearly changing post-induction feeding rate with a suitable slope allowed production of bioadhesive protein up to 5.3 g/L, which was higher than that obtained by the other post-induction feeding strategies. (C) 1998 John Wiley & Sons, Inc. | - |
dc.description.sponsorship | We thank Dr. Ina Goldberg for providing the E. coli strain. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | JOHN WILEY SONS INC | - |
dc.title | Effect of post-induction nutrient feeding strategies on the production of bioadhesive protein in Escherichia coli | - |
dc.type | Article | - |
dc.identifier.wosid | 000076088300001 | - |
dc.identifier.scopusid | 2-s2.0-0032487734 | - |
dc.type.rims | ART | - |
dc.citation.volume | 60 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 271 | - |
dc.citation.endingpage | 276 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.localauthor | Chang, Ho Nam | - |
dc.contributor.nonIdAuthor | Wong, HH | - |
dc.contributor.nonIdAuthor | Kim, YC | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | recombinant E-coli | - |
dc.subject.keywordAuthor | fed-batch | - |
dc.subject.keywordAuthor | protein production | - |
dc.subject.keywordAuthor | post-induction feeding | - |
dc.subject.keywordPlus | HIGH CELL-DENSITY | - |
dc.subject.keywordPlus | FED-BATCH CULTURES | - |
dc.subject.keywordPlus | RECOMBINANT PROTEIN | - |
dc.subject.keywordPlus | GROWTH-RATE | - |
dc.subject.keywordPlus | MYTILUS-EDULIS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PLASMID | - |
dc.subject.keywordPlus | GLUCOSE | - |
dc.subject.keywordPlus | ACETATE | - |
dc.subject.keywordPlus | CULTIVATION | - |
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