DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박, 태정 | - |
dc.contributor.author | 이, 상엽 | - |
dc.contributor.author | Park, T. J. | - |
dc.contributor.author | Lee, S. Y. | - |
dc.date.accessioned | 2010-11-24T01:46:28Z | - |
dc.date.available | 2010-11-24T01:46:28Z | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | Korean Journal of Biotechnology and Bioengineering, Vol.22, No.6, pp.371-377 | en |
dc.identifier.uri | http://hdl.handle.net/10203/20326 | - |
dc.description.abstract | Polyhydroxyalkanoates (PHAs) are a family of microbial polyesters that can be produced by fermentation from renewable resources. PHAs can be used as completely biodegradable plastics or elastomers. In this paper, novel applications of PHAs in biosensor are described. A general platform technology was developed by using the substrate binding domain (SBD) of PHA depolymerase as a fusion partner to immobilize proteins of interest on PHA surface. It could be shown that the proteins fused to the SBD of PHA depolymerase could be specifically immobilized onto PHA film, PHA microbead, and microcontact printed PHA surface. We review the results obtained for monitoring the specific interaction between the SBD and PHA by using enhanced green fluorescent protein, red fluorescent protein, single chain antibody against hepatitis B virus preS2 surface protein and severe acute respiratory syndrome coronavirus surface antigen as model proteins. Thus, this system can be efficiently used for studying protein-protein and possibly protein-biomolecule interactions for various biotechnological applications. | en |
dc.description.sponsorship | 본 논문은 정보통신부의 IT신성장동력 핵심기술 개발사 업 IT융합분야 연구과제와 초미세 화학공정 시스템 연구사 업에 의해 수행된 연구결과이며, 이에 감사드립니다. | en |
dc.language.iso | ko | en |
dc.publisher | 한국생물공학회 | en |
dc.subject | Poly(3-hydroxybutyrate) | en |
dc.subject | P(3HB) depolymerase | en |
dc.subject | substrate binding domain | en |
dc.subject | protein microarray | en |
dc.title | 생분해성 고분자, 폴리하이드록시알카노에이트를 이용한 바이오센서 칩 연구와 그 응용 | en |
dc.title.alternative | Research and Application of Polyhydroxyalkanoates in Biosensor Chip | en |
dc.type | Article | en |
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