Construction of Synthetic Promoter-Based Expression Cassettes for the Production of Cadaverine in Recombinant Corynebacterium glutamicum

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dc.contributor.authorOh, Young Hoonko
dc.contributor.authorChoi, Jae Wooko
dc.contributor.authorKim, Eun Youngko
dc.contributor.authorSong, Bong Keunko
dc.contributor.authorJeong, Ki Junko
dc.contributor.authorPark, Kyungmoonko
dc.contributor.authorKim, Il-Kwonko
dc.contributor.authorWoo, Han Minko
dc.contributor.authorLee, Seung Hwanko
dc.contributor.authorPark, Si Jaeko
dc.date.accessioned2016-04-12T07:08:41Z-
dc.date.available2016-04-12T07:08:41Z-
dc.date.created2015-09-01-
dc.date.created2015-09-01-
dc.date.created2015-09-01-
dc.date.issued2015-08-
dc.identifier.citationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.176, no.7, pp.2065 - 2075-
dc.identifier.issn0273-2289-
dc.identifier.urihttp://hdl.handle.net/10203/203149-
dc.description.abstractCorynebacterium glutamicum is an important microorganism in the biochemical industry for the production of various platform chemicals. However, despite its importance, a limited number of studies have been conducted on how to constitute gene expression cassettes in engineered C. glutamicum to obtain desired amounts of the target products. Therefore, in this study, six expression cassettes for the expression of the second lysine decarboxylase of Escherichia coli, LdcC, were constructed using six synthetic promoters with different strengths and were examined in C. glutamicum for the production of cadaverine. Among six expression cassettes, the expression of the E. coli ldcC gene under the PH30 promoter supported the highest production of cadaverine in flask and fed-batch cultivations. A fed-batch fermentation of recombinant C. glutamicum expressing E. coli ldcC gene under the PH30 promoter resulted in the production of 40.91 g/L of cadaverine in 64 h. This report is expected to contribute toward developing engineered C. glutamicum strains to have desired features.-
dc.languageEnglish-
dc.publisherHUMANA PRESS INC-
dc.titleConstruction of Synthetic Promoter-Based Expression Cassettes for the Production of Cadaverine in Recombinant Corynebacterium glutamicum-
dc.typeArticle-
dc.identifier.wosid000359432500020-
dc.identifier.scopusid2-s2.0-84939261206-
dc.type.rimsART-
dc.citation.volume176-
dc.citation.issue7-
dc.citation.beginningpage2065-
dc.citation.endingpage2075-
dc.citation.publicationnameAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY-
dc.identifier.doi10.1007/s12010-015-1701-4-
dc.contributor.localauthorJeong, Ki Jun-
dc.contributor.nonIdAuthorOh, Young Hoon-
dc.contributor.nonIdAuthorChoi, Jae Woo-
dc.contributor.nonIdAuthorKim, Eun Young-
dc.contributor.nonIdAuthorSong, Bong Keun-
dc.contributor.nonIdAuthorPark, Kyungmoon-
dc.contributor.nonIdAuthorKim, Il-Kwon-
dc.contributor.nonIdAuthorWoo, Han Min-
dc.contributor.nonIdAuthorLee, Seung Hwan-
dc.contributor.nonIdAuthorPark, Si Jae-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorCadaverine-
dc.subject.keywordAuthorL-lysine-
dc.subject.keywordAuthorFermentation-
dc.subject.keywordAuthorC. glutamicum-
dc.subject.keywordAuthorSynthetic promoters-
dc.subject.keywordPlusESCHERICHIA-COLI-
dc.subject.keywordPlusRENEWABLE RESOURCES-
dc.subject.keywordPlusPLATFORM CHEMICALS-
dc.subject.keywordPlusACID-
dc.subject.keywordPlus5-AMINOVALERATE-
dc.subject.keywordPlusPATHWAY-
dc.subject.keywordPlusNYLON-
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CBE-Journal Papers(저널논문)
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