DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yang, Taek Ho | ko |
dc.contributor.author | Kim, Tae Wan | ko |
dc.contributor.author | Kang, Hye Ok | ko |
dc.contributor.author | Lee, Sang-Hyun | ko |
dc.contributor.author | Lee, Eurn Jeong | ko |
dc.contributor.author | Lim, Sung-Chul | ko |
dc.contributor.author | Oh, Sun Ok | ko |
dc.contributor.author | Song, Ae-Jin | ko |
dc.contributor.author | Park, Si Jae | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2010-11-23T02:30:41Z | - |
dc.date.available | 2010-11-23T02:30:41Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2010-01 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOENGINEERING, v.105, no.1, pp.150 - 160 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | http://hdl.handle.net/10203/20255 | - |
dc.description.abstract | For the synthesis of polylactic acid (PLA) and its copolymers by one-step fermentation process, heterologous pathways involving Clostridium propionicum propionate CoA transferase (Pct(Cp)) and Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate (PHA) synthase I (PhaC1(Ps6-19)) were introduced into Escherichia coli for the generation of lactyl-CoA endogenously and incorporation of lactyl-CoA into the polymer, respectively. Since the wild-type PhaC1(Ps6-19) did not efficiently accept lactyl-CoA as a substrate, site directed mutagenesis as well as saturation mutagenesis were performed to improve the enzyme. The wild-type Pct(CP) was not able to efficiently convert lactate to lactyl-CoA and was found to exert inhibitory effect on cell growth, random mutagenesis by error-prone PCR was carried out. By employing engineered PhaC1(Ps6-19) and Pct(Cp), poly(3-hydroxybutyrate-co-lactate), P(3HB-co-LA), containing 20-49mol% lactate could be produced up to 62wt% from glucose and 3HB. By controlling the 3HB concentration in the medium, PLA homopolymer and P(3HB-co-LA) containing lactate as a major monomer unit could be synthesized. Also, P(3HB-co-LA) copolymers containing various lactate fractions could be produced from glucose alone by introducing the Cupriavidus necator beta-ketothiolase and acetoacetyl-CoA reductase genes. Fed-batch cultures were performed to produce P(3HB-co-LA) copolymers having 9-64 mol% of lactate, and their molecular weights, thermal properties, and melt flow properties were determined. Biotechnol. Bioeng. 2010;105: 150-160. (C) 2009 Wiley Periodicals, Inc. | - |
dc.description.sponsorship | We thank Yu Kyung Jung (KAIST) for her helpful discussion. Further supports by LG Chem Chair Professorship and Microsoft are appreciated. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | JOHN WILEY SONS INC | - |
dc.title | Biosynthesis of Polylactic Acid and Its Copolymers Using Evolved Propionate CoA Transferase and PHA Synthase | - |
dc.type | Article | - |
dc.identifier.wosid | 000272656200016 | - |
dc.identifier.scopusid | 2-s2.0-73949105231 | - |
dc.type.rims | ART | - |
dc.citation.volume | 105 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 150 | - |
dc.citation.endingpage | 160 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.identifier.doi | 10.1002/bit.22547 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Yang, Taek Ho | - |
dc.contributor.nonIdAuthor | Kim, Tae Wan | - |
dc.contributor.nonIdAuthor | Kang, Hye Ok | - |
dc.contributor.nonIdAuthor | Lee, Sang-Hyun | - |
dc.contributor.nonIdAuthor | Lee, Eurn Jeong | - |
dc.contributor.nonIdAuthor | Lim, Sung-Chul | - |
dc.contributor.nonIdAuthor | Oh, Sun Ok | - |
dc.contributor.nonIdAuthor | Park, Si Jae | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | polylactic acid | - |
dc.subject.keywordAuthor | PLA | - |
dc.subject.keywordAuthor | PHA synthase | - |
dc.subject.keywordAuthor | propionate CoA transferase | - |
dc.subject.keywordAuthor | enzyme evolution | - |
dc.subject.keywordPlus | RECOMBINANT ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | POLYHYDROXYALKANOATE SYNTHASES | - |
dc.subject.keywordPlus | SUBSTRATE-SPECIFICITY | - |
dc.subject.keywordPlus | POLY(LACTIC ACID) | - |
dc.subject.keywordPlus | MOLECULAR-WEIGHT | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | POLY(3-HYDROXYBUTYRATE) | - |
dc.subject.keywordPlus | POLYESTERS | - |
dc.subject.keywordPlus | ENZYME | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.