Novel strategy for production of aggregation-prone proteins and lytic enzymes in Escherichia coli based on an anchored periplasmic expression system

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For over 2 decades, Escherichia colt has been successfully used for the production of various recombinant proteins. However, several technical limitations have influenced the extent of recombinant protein expression in the E. coil host because of (i) heterologous protein accumulation often observed in inactive inclusion bodies either in the cytoplasm or periplasm, or (ii) lytic activity of recombinant proteins, which causes cell lysis, that hinder high production yield. We developed a novel strategy for the efficient production of aggregation-prone proteins and lytic enzymes in the E. coil host. For this purpose, we used an anchored periplasmic expression (APEx) system, in which target proteins are produced in the periplasm and tethered on the inner membrane. Protein aggregation and lytic activity can be prevented through anchoring of individual proteins to the inner membrane. Two model proteins (aggregation-prone human leptin and lytic Pseudomonas fluorescens SIK W1 lipase) were examined, and both proteins were successfully produced and anchored to the inner membrane under optimized culture conditions. Upon expression, the inner membrane-anchored proteins were subjected to simple purification procedures; the proteins were confirmed to be of high purity and bioactivity.
Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
Issue Date
2013-11
Language
English
Article Type
Article
Keywords

MALTOSE-BINDING PROTEIN; RECOMBINANT PROTEINS; INCLUSION-BODIES; LOW-TEMPERATURES; OSMOTIC SHOCK; HUMAN LEPTIN; IN-VIVO; LIPASE; PURIFICATION; GENE

Citation

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, v.116, no.5, pp.638 - 643

ISSN
1389-1723
DOI
10.1016/j.jbiosc.2013.04.023
URI
http://hdl.handle.net/10203/201297
Appears in Collection
CBE-Journal Papers(저널논문)
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