DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung, YK | ko |
dc.contributor.author | Park, Hyun Gyu | ko |
dc.contributor.author | Kim, JM | ko |
dc.date.accessioned | 2010-11-18T08:05:54Z | - |
dc.date.available | 2010-11-18T08:05:54Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2006-02 | - |
dc.identifier.citation | BIOSENSORS BIOELECTRONICS, v.21, no.8, pp.1536 - 1544 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | http://hdl.handle.net/10203/20128 | - |
dc.description.abstract | The natural binding of streptavidin (STA) for a small molecule, biotin, has made it a useful tool in specific targeting application, due to their most specific noncovalent biological interactions in nature. Here, we describe a polydiacetylene (PDA)-based colorimetric biosensor to detect the STA-biotin interactions. We synthesized two kinds of biotin-labeled 10,12-pentacosadiynoic acid (PCDA) monomers having 2(ethylene oxide) or 3(ethylene oxide) spacer and prepared biotin-modified PDA liposomes using them. Upon the addition of STA, the modified PDA liposomes showed a color change from blue to red in about I h followed by their aggregation and precipitation. The aggregation was found to be due to the cross-linking between the PDA liposomes caused by the STA having four biotin binding sites, which was confirmed by their TEM analysis. Since the STA-biotin interactions are widely adopted for various biological detection systems; the biotin-modified PDA sensor developed in this study has enormous potentials for the development of various colorimetric biosensors. (c) 2005 Elsevier B.V. All rights reserved. | - |
dc.description.sponsorship | We thankKorea Basic Science Institute for assistance with TEM experiments. Also, this research was supported by the Brain Korea 21 (BK 21) and Center for Ultramicrochemical Process Systems (CUPS) projects. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.subject | MOLECULAR RECOGNITION | - |
dc.subject | POLYDIACETYLENE VESICLES | - |
dc.subject | INTERFACIAL CATALYSIS | - |
dc.subject | LIPID VESICLES | - |
dc.subject | POLYMERIZATION | - |
dc.subject | BIOSENSORS | - |
dc.subject | FILMS | - |
dc.subject | NANOCOMPOSITES | - |
dc.subject | NANOPARTICLES | - |
dc.subject | LIPOSOMES | - |
dc.title | Polydiacelylene (PDA)-based colorimetric detection of biotin-streptavidin interactions | - |
dc.type | Article | - |
dc.identifier.wosid | 000235492900017 | - |
dc.identifier.scopusid | 2-s2.0-31044450823 | - |
dc.type.rims | ART | - |
dc.citation.volume | 21 | - |
dc.citation.issue | 8 | - |
dc.citation.beginningpage | 1536 | - |
dc.citation.endingpage | 1544 | - |
dc.citation.publicationname | BIOSENSORS BIOELECTRONICS | - |
dc.identifier.doi | 10.1016/j.bios.2005.07.010 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Park, Hyun Gyu | - |
dc.contributor.nonIdAuthor | Jung, YK | - |
dc.contributor.nonIdAuthor | Kim, JM | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | polydiacetylene | - |
dc.subject.keywordAuthor | biotin | - |
dc.subject.keywordAuthor | streptavidin | - |
dc.subject.keywordAuthor | colorimetric biosensors | - |
dc.subject.keywordPlus | MOLECULAR RECOGNITION | - |
dc.subject.keywordPlus | POLYDIACETYLENE VESICLES | - |
dc.subject.keywordPlus | INTERFACIAL CATALYSIS | - |
dc.subject.keywordPlus | LIPID VESICLES | - |
dc.subject.keywordPlus | POLYMERIZATION | - |
dc.subject.keywordPlus | BIOSENSORS | - |
dc.subject.keywordPlus | FILMS | - |
dc.subject.keywordPlus | NANOCOMPOSITES | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | LIPOSOMES | - |
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