DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Si Jae | ko |
dc.contributor.author | Oh, Young Hoon | ko |
dc.contributor.author | Noh, Won | ko |
dc.contributor.author | Kim, Hye Young | ko |
dc.contributor.author | Shin, Jae-Ho | ko |
dc.contributor.author | Lee, Eun Gyo | ko |
dc.contributor.author | Lee, Seungwoon | ko |
dc.contributor.author | David, Yokimiko | ko |
dc.contributor.author | Baylon, Mary Grace | ko |
dc.contributor.author | Song, Bong Keun | ko |
dc.contributor.author | Jegal, Jonggeon | ko |
dc.contributor.author | Lee, Sang-Yup | ko |
dc.contributor.author | Lee, Seung Hwan | ko |
dc.date.accessioned | 2015-11-20T09:51:46Z | - |
dc.date.available | 2015-11-20T09:51:46Z | - |
dc.date.created | 2014-12-24 | - |
dc.date.created | 2014-12-24 | - |
dc.date.created | 2014-12-24 | - |
dc.date.issued | 2014-10 | - |
dc.identifier.citation | BIOTECHNOLOGY JOURNAL, v.9, no.10, pp.1322 - 1328 | - |
dc.identifier.issn | 1860-6768 | - |
dc.identifier.uri | http://hdl.handle.net/10203/201134 | - |
dc.description.abstract | L-Lysine is a potential feedstock for the production of bio-based precursors for engineering plastics. In this study, we developed a microbial process for high-level conversion of L-lysine into 5-aminovalerate (5AVA) that can be used as a monomer in nylon 6,5 synthesis. Recombinant Escherichia coli WL3110 strain expressing Pseudomonas putida delta-aminovaleramidase (DavA) and lysine 2-monooxygenase (DavB) was grown to high density in fed-batch culture and used as a whole cell catalyst. High-density E. coli WL3110 expressing DavAB, grown to an optical density at 600 nm (OD600) of 30, yielded 36.51 g/L 5AVA from 60 g/L L-lysine in 24 h. Doubling the cell density of E. coli WL3110 improved the conversion yield to 47.96 g/L 5AVA from 60 g/L of L-lysine in 24 h. 5AVA production was further improved by doubling the L-lysine concentration from 60 to 120 g/L. The highest 5AVA titer (90.59 g/L; molar yield 0.942) was obtained from 120 g/L L-lysine by E. coli WL3110 cells grown to OD600 of 60. Finally, nylon 6,5 was synthesized by bulk polymerization of e-caprolactam and delta-valerolactam prepared from microbially synthesized 5AVA. The hybrid system demonstrated here has promising possibilities for application in the development of industrial bio-nylon production processes. | - |
dc.language | English | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | High-level conversion of L-lysine into 5-aminovalerate that can be used for nylon 6,5 synthesis | - |
dc.type | Article | - |
dc.identifier.wosid | 000342809300012 | - |
dc.identifier.scopusid | 2-s2.0-84908477634 | - |
dc.type.rims | ART | - |
dc.citation.volume | 9 | - |
dc.citation.issue | 10 | - |
dc.citation.beginningpage | 1322 | - |
dc.citation.endingpage | 1328 | - |
dc.citation.publicationname | BIOTECHNOLOGY JOURNAL | - |
dc.identifier.doi | 10.1002/biot.201400156 | - |
dc.contributor.localauthor | Lee, Sang-Yup | - |
dc.contributor.nonIdAuthor | Park, Si Jae | - |
dc.contributor.nonIdAuthor | Oh, Young Hoon | - |
dc.contributor.nonIdAuthor | Noh, Won | - |
dc.contributor.nonIdAuthor | Kim, Hye Young | - |
dc.contributor.nonIdAuthor | Lee, Eun Gyo | - |
dc.contributor.nonIdAuthor | Lee, Seungwoon | - |
dc.contributor.nonIdAuthor | David, Yokimiko | - |
dc.contributor.nonIdAuthor | Baylon, Mary Grace | - |
dc.contributor.nonIdAuthor | Song, Bong Keun | - |
dc.contributor.nonIdAuthor | Jegal, Jonggeon | - |
dc.contributor.nonIdAuthor | Lee, Seung Hwan | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | 5-Aminovaleric acid | - |
dc.subject.keywordAuthor | Bioconversion | - |
dc.subject.keywordAuthor | L-Lysine | - |
dc.subject.keywordAuthor | Nylon 6,5 | - |
dc.subject.keywordAuthor | Valerolactam | - |
dc.subject.keywordPlus | CORYNEBACTERIUM-GLUTAMICUM | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | SYSTEMS BIOLOGY | - |
dc.subject.keywordPlus | FERMENTATION | - |
dc.subject.keywordPlus | CADAVERINE | - |
dc.subject.keywordPlus | CATABOLISM | - |
dc.subject.keywordPlus | GLUTARATE | - |
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