Lpg0393 of Legionella pneumophila Is a Guanine-Nucleotide Exchange Factor for Rab5, Rab21 and Rab22

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Legionella pneumophila, a human intracellular pathogen, encodes about 290 effector proteins that are translocated into host cells through a secretion machinery. Some of these proteins have been shown to manipulate or subvert cellular processes during infection, but functional roles of a majority of them remain unknown. Lpg0393 is a newly identified Legionella effector classified as a hypothetical protein. Through X-ray crystallographic analysis, we show that Lpg0393 contains a Vps9-like domain, which is structurally most similar to the catalytic core of human Rabex-5 that activates the endosomal Rab proteins Rab5, Rab21 and Rab22. Consistently, Lpg0393 exhibited a guanine-nucleotide exchange factor activity toward the endosomal Rabs. This work identifies the first example of a bacterial guanine-nucleotide exchange factor that is active towards the Rab5 sub-cluster members, implying that the activation of these Rab proteins might be advantageous for the intracellular survival of Legionella.
Publisher
PUBLIC LIBRARY SCIENCE
Issue Date
2015-03
Language
English
Article Type
Article
Keywords

EFFECTOR PROTEIN DRRA; STRUCTURAL BASIS; PLASMA-MEMBRANE; GTPASE FUNCTION; X-RAY; CELL; ACTIVATION; RABEX-5; SYSTEM; VPS9

Citation

PLOS ONE, v.10, no.3

ISSN
1932-6203
DOI
10.1371/journal.pone.0118683
URI
http://hdl.handle.net/10203/201010
Appears in Collection
BS-Journal Papers(저널논문)
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