DC Field | Value | Language |
---|---|---|
dc.contributor.author | Seok, Seunghwan | ko |
dc.contributor.author | Shin, Sujeong | ko |
dc.contributor.author | Lee, Tae Jae | ko |
dc.contributor.author | Jeong, Jae-Min | ko |
dc.contributor.author | Yang, MinHo | ko |
dc.contributor.author | Kim, Do-Hyun | ko |
dc.contributor.author | Park, Jung Youn | ko |
dc.contributor.author | Lee, Seok Jae | ko |
dc.contributor.author | Choi, Bong Gill | ko |
dc.contributor.author | Lee, Kyoung G. | ko |
dc.date.accessioned | 2015-11-20T09:06:09Z | - |
dc.date.available | 2015-11-20T09:06:09Z | - |
dc.date.created | 2015-05-12 | - |
dc.date.created | 2015-05-12 | - |
dc.date.created | 2015-05-12 | - |
dc.date.issued | 2015-03 | - |
dc.identifier.citation | ACS APPLIED MATERIALS & INTERFACES, v.7, no.8, pp.4699 - 4705 | - |
dc.identifier.issn | 1944-8244 | - |
dc.identifier.uri | http://hdl.handle.net/10203/201008 | - |
dc.description.abstract | Selective filtering of target biomaterials from impurities is an important task in DNA amplification through polymerase chain reaction (PCR) enhancement and gene identification to save endangered animals and marine species. Conventional gene extraction methods require complicated steps, skilled persons, and expensive chemicals and instruments to improve DNA amplification. Herein, we proposed an alternative method for overcoming such challenges by imparting secondary functionality using commercially available polyurethane (PU) sponges and cost-effective fabrication approaches through polydopamine and polysiloxane coatings. The porous, highly flexible, and chemically modified superhydrophilic and superhydrophobic PU sponges allow large surface areas and mechanically stable frames for effective extraction of genomic DNA through selective filtering of fish tissues and oils. Furthermore, these chemically modified PU sponges allow separation of genes and improvement of PCR for DNA amplification for the identification of fish species. The combination of a simple fabrication method and functionalized PU sponges could be a useful platform for PCR enhancement and gene-based identification of species for practical applications. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Multifunctional Polyurethane Sponge for Polymerase Chain Reaction Enhancement | - |
dc.type | Article | - |
dc.identifier.wosid | 000353005300031 | - |
dc.identifier.scopusid | 2-s2.0-84924241246 | - |
dc.type.rims | ART | - |
dc.citation.volume | 7 | - |
dc.citation.issue | 8 | - |
dc.citation.beginningpage | 4699 | - |
dc.citation.endingpage | 4705 | - |
dc.citation.publicationname | ACS APPLIED MATERIALS & INTERFACES | - |
dc.identifier.doi | 10.1021/am508101m | - |
dc.contributor.localauthor | Kim, Do-Hyun | - |
dc.contributor.nonIdAuthor | Shin, Sujeong | - |
dc.contributor.nonIdAuthor | Lee, Tae Jae | - |
dc.contributor.nonIdAuthor | Yang, MinHo | - |
dc.contributor.nonIdAuthor | Park, Jung Youn | - |
dc.contributor.nonIdAuthor | Lee, Seok Jae | - |
dc.contributor.nonIdAuthor | Choi, Bong Gill | - |
dc.contributor.nonIdAuthor | Lee, Kyoung G. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | polyurethane | - |
dc.subject.keywordAuthor | filter | - |
dc.subject.keywordAuthor | superhydrophobic | - |
dc.subject.keywordAuthor | superhydrophilic | - |
dc.subject.keywordAuthor | PCR | - |
dc.subject.keywordAuthor | DNA | - |
dc.subject.keywordPlus | SUPERHYDROPHOBIC SURFACES | - |
dc.subject.keywordPlus | OIL/WATER SEPARATION | - |
dc.subject.keywordPlus | WATER | - |
dc.subject.keywordPlus | FABRICATION | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | EXTRACTION | - |
dc.subject.keywordPlus | CHEMISTRY | - |
dc.subject.keywordPlus | MECHANISM | - |
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