Transcriptomic analysis of Corynebacterium glutamicum in the response to the toxicity of furfural present in lignocellulosic hydrolysates

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dc.contributor.authorPark, Hong-Silko
dc.contributor.authorUm, Youngsoonko
dc.contributor.authorSim, Sang Junko
dc.contributor.authorLee, Sang-Yupko
dc.contributor.authorWoo, Han Minko
dc.date.accessioned2015-11-20T07:37:06Z-
dc.date.available2015-11-20T07:37:06Z-
dc.date.created2015-05-06-
dc.date.created2015-05-06-
dc.date.created2015-05-06-
dc.date.issued2015-03-
dc.identifier.citationPROCESS BIOCHEMISTRY, v.50, no.3, pp.347 - 356-
dc.identifier.issn1359-5113-
dc.identifier.urihttp://hdl.handle.net/10203/200777-
dc.description.abstractPretreatment of lignocellulosic biomass by acid hydrolysis generates growth inhibitors such as furfural, 5-hydroxymethylfurfural, and acetic acid. Among the inhibitors, furfural strongly inhibits cell growth, our objective was to identify the furfural stimulon of an amino acid producer Corynebacterium glutamicum using transcriptomic analysis. As a result, 182 up-regulated (2-fold) genes and 81 down-regulated (0.5-fold) genes were identified for all furfural stress conditions (6.5 mM, 13 mM, and 20 mM). Based on the functional categories of CoryneRegNet database, the furfural stimulon was related to genetic responses of oxidative stress, SOS responses, and anaerobic respiratory metabolism. To overcome the furfural toxicity, furfural-responsive efflux-like permease encoded by the cg1661 gene was overexpressed, resulting that the optical density at 600nm was 1.54-fold enhanced over the control at 12 h. This study provides alternative strategy of metabolic engineering for biological detoxification in addition to overexpression of NADH-dependent reductases.-
dc.languageEnglish-
dc.publisherELSEVIER SCI LTD-
dc.titleTranscriptomic analysis of Corynebacterium glutamicum in the response to the toxicity of furfural present in lignocellulosic hydrolysates-
dc.typeArticle-
dc.identifier.wosid000352745300004-
dc.identifier.scopusid2-s2.0-84923332249-
dc.type.rimsART-
dc.citation.volume50-
dc.citation.issue3-
dc.citation.beginningpage347-
dc.citation.endingpage356-
dc.citation.publicationnamePROCESS BIOCHEMISTRY-
dc.identifier.doi10.1016/j.procbio.2014.11.014-
dc.contributor.localauthorLee, Sang-Yup-
dc.contributor.nonIdAuthorPark, Hong-Sil-
dc.contributor.nonIdAuthorUm, Youngsoon-
dc.contributor.nonIdAuthorSim, Sang Jun-
dc.contributor.nonIdAuthorWoo, Han Min-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorCorynebacterium glutamicum-
dc.subject.keywordAuthorFurfural-
dc.subject.keywordAuthorEfflux-like permease-
dc.subject.keywordAuthorDNA microarray-
dc.subject.keywordAuthorMetabolic engineering-
dc.subject.keywordPlusETHANOLOGENIC ESCHERICHIA-COLI-
dc.subject.keywordPlusSACCHAROMYCES-CEREVISIAE-
dc.subject.keywordPlusOXIDATIVE STRESS-
dc.subject.keywordPlusGENES-
dc.subject.keywordPlusTOLERANCE-
dc.subject.keywordPlusRESISTANCE-
dc.subject.keywordPlusINHIBITORS-
dc.subject.keywordPlusGROWTH-
dc.subject.keywordPlusOVEREXPRESSION-
dc.subject.keywordPlusIDENTIFICATION-
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CBE-Journal Papers(저널논문)
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