Production of recombinant proteins by high cell density culture of Escherichia coli

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Escherichia coli has been the most widely used host for the production of recombinant proteins because it is the best characterized system in every aspect. Furthermore, the high cell density culture of recombinant E. coli has allowed production of various proteins with high yield and high productivities. Various cultivation strategies employing different host strains and expression systems have been successfully employed for the production of recombinant proteins. New strategies for strain improvement towards the goal of enhanced protein production are actively being developed based on high-throughput omics approaches such as transcriptomics and proteomcs. This paper reviews recent advances in the production of recombinant proteins by high cell density culture of E. coli. (c) 2005 Elsevier Ltd. All rights reserved.
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Issue Date
2006-02
Language
English
Article Type
Review
Keywords

SINGLE-STEP PURIFICATION; COLONY-STIMULATING FACTOR; EPIDERMAL-GROWTH-FACTOR; HIGH-LEVEL EXPRESSION; FED-BATCH CULTURE; SECRETORY PRODUCTION; HETEROLOGOUS PROTEINS; ENHANCED PRODUCTION; INCLUSION-BODIES; HUMAN LEPTIN

Citation

CHEMICAL ENGINEERING SCIENCE, v.61, pp.876 - 885

ISSN
0009-2509
DOI
10.1016/j.ces.2005.03.031
URI
http://hdl.handle.net/10203/19918
Appears in Collection
CBE-Journal Papers(저널논문)
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