Fluorescence-based assay formats and signal amplification strategies for DNA microarray analysis

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DNA rnicroarrays are powerful tools for the high throughput analysis of nucleic acids due to their parallel detection capabilities. To realize the apparent power of DNA microarray, an efficient assay format is essential and a variety of assay formats have been developed for nucleic acid detection on microarrays. Many of them employ fluorescence-based methods because fluorescence detection is straightforward and easy to implement. Herein we broadly review fluorescence-based assay formats with a focus on PCR-associated target preparation. For the heterogeneous assay of nucleic acid on DNA microarrays, sensitivity is one of the most important factors and special emphasis has been given to recently developed signal amplification strategies aimed at achieving high sensitivity. (c) 2005 Elsevier Ltd. All rights reserved.
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Issue Date
2006-02
Language
English
Article Type
Review
Keywords

DENSITY OLIGONUCLEOTIDE ARRAYS; ROLLING-CIRCLE AMPLIFICATION; GENE-EXPRESSION ANALYSIS; IN-SITU HYBRIDIZATION; MUTATION DETECTION; MESSENGER-RNA; NUCLEIC-ACIDS; INFECTIOUS-DISEASES; MASS-SPECTROMETRY; SURFACE

Citation

CHEMICAL ENGINEERING SCIENCE, v.61, no.3, pp.954 - 965

ISSN
0009-2509
DOI
10.1016/j.ces.2005.05.054
URI
http://hdl.handle.net/10203/19894
Appears in Collection
CBE-Journal Papers(저널논문)
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