Simple synthesis of hierarchically ordered mesocellular mesoporous silica materials hosting crosslinked enzyme aggregates

Cited 174 time in webofscience Cited 0 time in scopus
  • Hit : 317
  • Download : 10
Hierarchically ordered mesocellular mesoporous silica materials (HMMS) were synthesized using a single structure-directing agent. The mesocellular pores are synthesized without adding any pore expander; the pore walls are composed of SBA-15 type mesopores. Small-angle X-ray scattering revealed the presence of uniform' pore structures with two different sizes. Using HMMS as a nanoscopic template, hierarchically ordered mesocellular mesoporous carbon (HMMC) and polymer (HMMP) materials were synthesized. HMMS was used as a host for enzyme immobilization. To improve the retention of enzymes in HMMS, we adsorbed enzymes, and then employed crosslinking using glutaraldehyde (GA). The resulting crosslinked enzyme aggregates (CLEAs) show an impressive stability with extremely high enzyme loadings. For example, 0.5 g alpha-chymotrypsin (CT) could be loaded in 1 g of silica with no activity decrease observed with rigorous shaking over one month. In contrast, adsorbed CT without GA treatment resulted in a lower loading, which further decreased due to continuous leaching of adsorbed CT under shaking. The activity of crosslinked CT aggregates in HMMS was approximate to 10 times higher than that of the adsorbed CT, which represents a 74-fold increase in activity per unit weight of HMMS due to higher CT loading.
Publisher
WILEY-V C H VERLAG GMBH
Issue Date
2005-07
Language
English
Article Type
Article
Keywords

CHEMICAL-VAPOR-DEPOSITION; CARBON MOLECULAR-SIEVES; CAGE-LIKE PORES; POROUS-SILICA; METAL-OXIDES; TRIBLOCK COPOLYMER; NANOPOROUS CARBON; POLYMER FOAMS; LOW-COST; TEMPLATES

Citation

SMALL, v.1, no.7, pp.744 - 753

ISSN
1613-6810
DOI
10.1002/smll.200500035
URI
http://hdl.handle.net/10203/19882
Appears in Collection
CBE-Journal Papers(저널논문)
Files in This Item
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 174 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0