DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, SJ | ko |
dc.contributor.author | Park, JP | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2010-11-12T07:37:34Z | - |
dc.date.available | 2010-11-12T07:37:34Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2002-09 | - |
dc.identifier.citation | FEMS MICROBIOLOGY LETTERS, v.214, no.2, pp.217 - 222 | - |
dc.identifier.issn | 0378-1097 | - |
dc.identifier.uri | http://hdl.handle.net/10203/19842 | - |
dc.description.abstract | The Escherichia coli fabG(Ec) gene and the Pseudomonas aeruginosa rhlG(Pa) gene, which encode 3-ketoacyl-acyl carrier protein reductase, were expressed in E. coli W3110 and its fadA mutant strain WA101 to examine their roles in medium-chain-length (MCL) polyhydroxyalkanoate (PHA) biosynthesis from fatty acids. When one of these 3-ketoacyl-acyl carrier protein reductase genes was co-expressed with the Pseudomonas sp. 61-3 PHA synthase gene (phaC2(Ps)) in E. coli W3110, MCL-PHA composed mainly of 3-hydroxyoctanoate and 3-hydroxydecanoate was synthesized from sodium decanoate. When the fabG(Ec) gene and the phaC2(Ps) gene were co-expressed in the fadA mutant E. coli strain WA101, MCL-PHA rich in 3-hydroxydecanoate monomer up to 93 mol% was accumulated from sodium decanoate. This was possible by efficiently redirecting 3-ketoacyl-coenzymes A from the beta-oxidation pathway to the PHA biosynthesis pathway without losing two carbon units, the strategy of which can be extended for the production of MCL-PHAs rich in other specific monomers. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. | - |
dc.description.sponsorship | This work was supported by the National Research Laboratory Program (2000-N-NL-01-C-237) of the Korean Ministry of Science and Technology (MOST). We thank Dr. Y. Doi and Dr. Isabelle-S. Hinner for kindly providing us with plasmid pBSEB50 and pBBR1MCS, respectively. We also thank Dr. G.M. Church for the kind gift of plasmid pKO3. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Metabolic engineering of Escherichia coli for the production of medium-chain-length polyhydroxyalkanoates rich in specific monomers | - |
dc.type | Article | - |
dc.identifier.wosid | 000178467500013 | - |
dc.identifier.scopusid | 2-s2.0-0037057135 | - |
dc.type.rims | ART | - |
dc.citation.volume | 214 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 217 | - |
dc.citation.endingpage | 222 | - |
dc.citation.publicationname | FEMS MICROBIOLOGY LETTERS | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Park, SJ | - |
dc.contributor.nonIdAuthor | Park, JP | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | polyhydroxyalkanoate | - |
dc.subject.keywordAuthor | beta-oxidation pathway | - |
dc.subject.keywordAuthor | 3-ketoacyl-acyl carrier protein reductase | - |
dc.subject.keywordAuthor | Escherichia coli FabG | - |
dc.subject.keywordAuthor | Pseudomonas aeruginosa RhlG | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.subject.keywordPlus | COMPLETE GENOME SEQUENCE | - |
dc.subject.keywordPlus | PSEUDOMONAS-AERUGINOSA | - |
dc.subject.keywordPlus | REDUCTASE | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | PHAC1 | - |
dc.subject.keywordPlus | ACID | - |
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