DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung, Jaehwan | ko |
dc.contributor.author | Park, Byung Hyun | ko |
dc.contributor.author | Oh, Seung Jun | ko |
dc.contributor.author | Choi, Goro | ko |
dc.contributor.author | Seo, Tae-Seok | ko |
dc.date.accessioned | 2015-04-29T01:03:56Z | - |
dc.date.available | 2015-04-29T01:03:56Z | - |
dc.date.created | 2015-04-21 | - |
dc.date.created | 2015-04-21 | - |
dc.date.issued | 2015-06 | - |
dc.identifier.citation | BIOSENSORS & BIOELECTRONICS, v.68, pp.218 - 224 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | http://hdl.handle.net/10203/198207 | - |
dc.description.abstract | An integrated reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) microdevice which consists of microbead-assisted RNA purification and RT-LAMP with real-time monitoring by a miniaturized optical detector was demonstrated. The integrated RT-LAMP microdevice includes four reservoirs for a viral RNA sample (purified influenza A viral RNA or lysates), a washing solution (70% ethanol), an elution solution (RNase-free water), and an RT-LAMP cocktail, and two chambers (a waste chamber and an RT-LAMP reaction chamber). The separate reservoirs for a washing solution, an elution solution, and an RT-LAMP cocktail were designed with capillary valves for stable storage. Three influenza A virus strains (A/H1N1, A/H3N2, and A/H5N1) were used for RNA templates, and RT-LAMP primer sets were designed to detect hemagglutinin (HA) and conserved M gene. Sequential sample flow to the microbeads for RNA purification was achieved by centrifugal force with optimization of capillary valves and a siphon channel. Furthermore, the purified RNA solution was successfully isolated from the waste solution by changing the rotational direction, and combined with the RT-LAMP cocktail in the RT-LAMP reaction chamber for target gene amplification. Total process from the sample injection to the result was completed in 47 min. Influenza A H1N1 virus was confirmed on the integrated RT-LAMP microdevice even with 10 copies of viral RNAs, which revealed 10-fold higher sensitivity than that of a conventional RT-PCR. Subtyping and specificity test of influenza A H1N1 viral lysates were also performed and clinical samples were successfully genotyped to confirm influenza A virus on our proposed integrated microdevice. | - |
dc.language | English | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.subject | RAPID DETECTION | - |
dc.subject | WHOLE-BLOOD | - |
dc.subject | MICROFLUIDIC PLATFORM | - |
dc.subject | SAMPLE PRETREATMENT | - |
dc.subject | DNA AMPLIFICATION | - |
dc.subject | REAL-TIME | - |
dc.subject | EXTRACTION | - |
dc.subject | SYSTEM | - |
dc.subject | DEVICE | - |
dc.subject | LAMP | - |
dc.title | Integrated centrifugal reverse transcriptase loop-mediated isothermal amplification microdevice for influenza A virus detection | - |
dc.type | Article | - |
dc.identifier.wosid | 000351248300031 | - |
dc.identifier.scopusid | 2-s2.0-84920401041 | - |
dc.type.rims | ART | - |
dc.citation.volume | 68 | - |
dc.citation.beginningpage | 218 | - |
dc.citation.endingpage | 224 | - |
dc.citation.publicationname | BIOSENSORS & BIOELECTRONICS | - |
dc.identifier.doi | 10.1016/j.bios.2014.12.043 | - |
dc.contributor.localauthor | Seo, Tae-Seok | - |
dc.contributor.nonIdAuthor | Choi, Goro | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Centrifugal microdevice | - |
dc.subject.keywordAuthor | Influenza A virus | - |
dc.subject.keywordAuthor | Integrated microdevice | - |
dc.subject.keywordAuthor | Reverse transcriptase loop-mediated ampliciation | - |
dc.subject.keywordAuthor | Real-time fluorescent detection | - |
dc.subject.keywordAuthor | Sample pretreatment | - |
dc.subject.keywordPlus | RAPID DETECTION | - |
dc.subject.keywordPlus | WHOLE-BLOOD | - |
dc.subject.keywordPlus | MICROFLUIDIC PLATFORM | - |
dc.subject.keywordPlus | SAMPLE PRETREATMENT | - |
dc.subject.keywordPlus | DNA AMPLIFICATION | - |
dc.subject.keywordPlus | REAL-TIME | - |
dc.subject.keywordPlus | EXTRACTION | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | DEVICE | - |
dc.subject.keywordPlus | LAMP | - |
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