DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박시재 | ko |
dc.contributor.author | 이승환 | ko |
dc.contributor.author | 오영훈 | ko |
dc.contributor.author | 이상엽 | ko |
dc.date.accessioned | 2015-04-08T05:35:50Z | - |
dc.date.available | 2015-04-08T05:35:50Z | - |
dc.date.created | 2015-03-20 | - |
dc.date.created | 2015-03-20 | - |
dc.date.created | 2015-03-20 | - |
dc.date.issued | 2014-08 | - |
dc.identifier.citation | KSBB JOURNAL, v.29, no.4, pp.244 - 249 | - |
dc.identifier.issn | 1225-7117 | - |
dc.identifier.uri | http://hdl.handle.net/10203/195711 | - |
dc.description.abstract | Biosynthesis pathway of medium-chain-length(MCL) polyhydroxyalkanoates (PHA) from fatty acid β-oxidationpathway was constructed in recombinant Escherichiacoli by introducing the Pseudomonas sp. 61-3 PHA synthasegene (phaC2) and the maoC genes from Pseudomonas putida,Sinorhizobium meliloti, and Ralstonia eutropha. The metaboliclink between fatty acid β-oxidation pathway and PHAbiosynthesis pathway was constructed by MaoC, which ishomologous to P. aeruginosa (R)-specific enoyl-CoA hydratase(PhaJ1). When the E. coli W3110 strains expressing thephaC2 gene and one of the maoC genes from P. putida, Sinorhizobiummeliloti, and Ralstonia eutropha were cultured inLB medium containing 2 g/L of sodium decanoate as a carbonsource, MCL-PHA that mainly consists of 3-hydroxyhexanoate(3HHx), 3-hydroxyoctanoate (3HO) and 3-hydroxydecanoate(3HD), was produced. The monomer composition ofPHA and PHA contents varied depending on MaoC employedfor the production of PHA. The highest PHA content of 18.7wt% was achieved in recombinant E. coli W3110 expressingthe phaC2 gene and the P. putida maoC gene. These resultssuggest that MCL-PHA biosynthesis pathway can be constructedin recombinant E. coli strains from the b-oxidation pathwayby employing MaoC able to supply (R)-3-hydroxyacyl-CoA, the substrate of PHA synthase. | - |
dc.language | Korean | - |
dc.publisher | 한국생물공학회 | - |
dc.title | 재조합 대장균에서 MaoC를 이용한 지방산으로부터의 중간사슬길이 폴리하이드록시알칸산 생산 연구 | - |
dc.title.alternative | MaoC Mediated Biosynthesis of Medium-chain-length Polyhydroxyalkanoates in Recombinant Escherichia coli from Fatty Acid | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.citation.volume | 29 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 244 | - |
dc.citation.endingpage | 249 | - |
dc.citation.publicationname | KSBB JOURNAL | - |
dc.identifier.kciid | ART001910690 | - |
dc.contributor.localauthor | 이상엽 | - |
dc.contributor.nonIdAuthor | 박시재 | - |
dc.contributor.nonIdAuthor | 이승환 | - |
dc.contributor.nonIdAuthor | 오영훈 | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.subject.keywordAuthor | MaoC | - |
dc.subject.keywordAuthor | β-oxidation | - |
dc.subject.keywordAuthor | Polyhydroxyalkanoates | - |
dc.subject.keywordAuthor | Fatty acid | - |
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