DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yim, Sung Sun | ko |
dc.contributor.author | An, Seul Ji | ko |
dc.contributor.author | Choi, Jae Woong | ko |
dc.contributor.author | Ryu, Ae Jin | ko |
dc.contributor.author | Jeong, Kijun | ko |
dc.date.accessioned | 2015-01-27T02:24:15Z | - |
dc.date.available | 2015-01-27T02:24:15Z | - |
dc.date.created | 2014-01-27 | - |
dc.date.created | 2014-01-27 | - |
dc.date.created | 2014-01-27 | - |
dc.date.issued | 2014-01 | - |
dc.identifier.citation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.98, no.1, pp.273 - 284 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | http://hdl.handle.net/10203/193112 | - |
dc.description.abstract | We describe the development of a new secretory production system for the enhanced production of a single-chain variable fragment (scFv) against the anthrax toxin in Corynebacterium glutamicum. For efficient secretory production of the antibody fragment, the following components were examined: (1) signal peptides, (2) codon usage of antibody fragment, (3) promoters, (4) 5' untranslated region (5' UTR) sequence, and (5) transcriptional terminator. Among all the systems examined, the use of a codon-optimized gene sequence, a Sec-dependent PorB signal peptide, and a fully synthetic H36 promoter, allowed the highest production of antibody fragments in a culture medium. For large-scale production, fed-batch cultivations were also conducted in a 5-L lab-scale bioreactor. When cells were cultivated in semi-defined media, cells could grow up to an OD600 of 179 for 32 h and an antibody fragment concentration as high as 68 mg/L could be obtained in a culture medium with high purity. From the culture medium, the secreted antibody was successfully purified using a simple purification procedure, with correct binding activity confirmed by enzyme-linked immunosorbent assay. To the best of our knowledge, this is the first report of a fed-batch cultivation for antibody fragment production in C. glutamicum. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.title | High-level secretory production of recombinant single-chain variable fragment (scFv) in Corynebacterium glutamicum | - |
dc.type | Article | - |
dc.identifier.wosid | 000329093600025 | - |
dc.identifier.scopusid | 2-s2.0-84891853096 | - |
dc.type.rims | ART | - |
dc.citation.volume | 98 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 273 | - |
dc.citation.endingpage | 284 | - |
dc.citation.publicationname | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.identifier.doi | 10.1007/s00253-013-5315-x | - |
dc.contributor.localauthor | Yim, Sung Sun | - |
dc.contributor.localauthor | Jeong, Kijun | - |
dc.contributor.nonIdAuthor | An, Seul Ji | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Corynebacterium glutamicum | - |
dc.subject.keywordAuthor | Secretion | - |
dc.subject.keywordAuthor | Antibody fragment | - |
dc.subject.keywordAuthor | Fed-batch cultivation | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | ANTIBODY-FRAGMENT | - |
dc.subject.keywordPlus | ENHANCED PRODUCTION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | LACTATE | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | GENES | - |
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