Autophagy and its implication in Chinese hamster ovary cell culture

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Chinese hamster ovary (CHO) cells, that are widely used for production of therapeutic proteins, are subjected to apoptosis and autophagy under the stresses induced by conditions such as nutrient deprivation, hyperosmolality and addition of sodium butyrate. To achieve a cost-effective level of production, it is important to extend the culture longevity. Until now, there have been numerous studies in which apoptosis of recombinant CHO (rCHO) cells was inhibited, resulting in enhanced production of therapeutic proteins. Recently, autophagy in rCHO cells has drawn attention because it can be genetically and chemically controlled to increase cell survival and productivity. Autophagy is a global catabolic process which involves multiple pathways and genes that regulate the lysosomal degradation of intracellular components. A simultaneous targeting of anti-apoptosis and pro-autophagy could lead to more efficient protection of cells from stressful culture conditions. In this regard, it is worthwhile to have a detailed understanding of the autophagic pathway, in order to select appropriate genes and chemical targets to manage autophagy in rCHO cells, and thus to enhance the production of therapeutic proteins.
Publisher
SPRINGER
Issue Date
2013-11
Language
English
Article Type
Review
Keywords

ACTIVATED PROTEIN-KINASE; ISOLATED RAT HEPATOCYTES; REGULATES AUTOPHAGY; CHO-CELLS; SODIUM-BUTYRATE; CANCER-THERAPY; BCL-X(L) OVEREXPRESSION; APOPTOSIS INHIBITION; MONOCLONAL-ANTIBODY; HUNTINGTONS-DISEASE

Citation

BIOTECHNOLOGY LETTERS, v.35, no.11, pp.1753 - 1763

ISSN
0141-5492
DOI
10.1007/s10529-013-1276-5
URI
http://hdl.handle.net/10203/189490
Appears in Collection
BS-Journal Papers(저널논문)
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