Effect of sodium butyrate on the assembly, charge variants, and galactosylation of antibody produced in recombinant Chinese hamster ovary cells

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Sodium butyrate (NaBu) is known to increase the specific productivity of recombinant Chinese hamster ovary (rCHO) cells. To understand the effects of NaBu on the product quality, rCHO cells producing monoclonal antibody (Mab) were cultivated at various concentrations of NaBu (0 to 4 mM). NaBu increased correctly assembled Mab. In the absence of NaBu, the proportions of intact Mab (2H2L) and heavy chain dimer (2H) were 81 and 15 %. At 1 mM NaBu, the proportion of 2H2L increased to 93 %, whereas the proportion of 2H decreased to 2 %. No further increase in the proportion of 2H2L was obtained at a higher NaBu concentration. NaBu also affected the charge heterogeneity of Mab, which may affect the efficacy of Mab. The basic charge variants of Mabs increased with an increase in the NaBu concentration. In addition, NaBu affected the galactosylation of Mab negatively. Overall, the data obtained here show that NaBu used in rCHO cell cultures for improved Mab production affects certain quality aspects of Mab, in this case, the charge heterogeneity and galactosylation.
Publisher
SPRINGER
Issue Date
2014-06
Language
English
Article Type
Article
Keywords

CHO-CELLS; ENDOPLASMIC-RETICULUM; LIGHT-CHAINS; HUMAN THROMBOPOIETIN; CULTURE-CONDITIONS; PROTEIN; IMMUNOGLOBULIN; HETEROGENEITY; GLYCOSYLATION; APOPTOSIS

Citation

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.98, no.12, pp.5417 - 5425

ISSN
0175-7598
DOI
10.1007/s00253-014-5596-8
URI
http://hdl.handle.net/10203/189412
Appears in Collection
BS-Journal Papers(저널논문)
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