DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yang, Jung Eun | ko |
dc.contributor.author | Choi, Yong-Jun | ko |
dc.contributor.author | Lee, Se Jin | ko |
dc.contributor.author | Kang, Kyoung-Hee | ko |
dc.contributor.author | Lee, Hyuk | ko |
dc.contributor.author | Oh, Young Hoon | ko |
dc.contributor.author | Lee, Seung Hwan | ko |
dc.contributor.author | Park, Si Jae | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2014-08-29T01:08:39Z | - |
dc.date.available | 2014-08-29T01:08:39Z | - |
dc.date.created | 2014-01-27 | - |
dc.date.created | 2014-01-27 | - |
dc.date.created | 2014-01-27 | - |
dc.date.issued | 2014-01 | - |
dc.identifier.citation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.98, no.1, pp.95 - 104 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | http://hdl.handle.net/10203/188696 | - |
dc.description.abstract | The Escherichia coli XL1-blue strain was metabolically engineered to synthesize poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] through 2-ketobutyrate, which is generated via citramalate pathway, as a precursor for propionyl-CoA. Two different metabolic pathways were examined for the synthesis of propionyl-CoA from 2-ketobutyrate. The first pathway is composed of the Dickeya dadantii 3937 2-ketobutyrate oxidase or the E. coli pyruvate oxidase mutant (PoxB L253F V380A) for the conversion of 2-ketobutyrate into propionate and the Ralstonia eutropha propionyl-CoA synthetase (PrpE) or the E. coli acetyl-CoA:acetoacetyl-CoA transferase for further conversion of propionate into propionyl-CoA. The second pathway employs pyruvate formate lyase encoded by the E. coli tdcE gene or the Clostridium difficile pflB gene for the direct conversion of 2-ketobutyrate into propionyl-CoA. As the direct conversion of 2-ketobutyrate into propionyl-CoA could not support the efficient production of P(3HB-co-3HV) from glucose, the first metabolic pathway was further examined. When the recombinant E. coli XL1-blue strain equipped with citramalate pathway expressing the E. coli poxB L253F V380A gene and R. eutropha prpE gene together with the R. eutropha PHA biosynthesis genes was cultured in a chemically defined medium containing 20 g/L of glucose as a sole carbon source, P(3HB-co-2.3 mol% 3HV) was produced up to the polymer content of 61.7 wt.%. Moreover, the 3HV monomer fraction in P(3HB-co-3HV) could be increased up to 5.5 mol% by additional deletion of the prpC and scpC genes, which are responsible for the metabolism of propionyl-CoA in host strains. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.title | Metabolic engineering of Escherichia coli for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from glucose | - |
dc.type | Article | - |
dc.identifier.wosid | 000329093600009 | - |
dc.identifier.scopusid | 2-s2.0-84891860323 | - |
dc.type.rims | ART | - |
dc.citation.volume | 98 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 95 | - |
dc.citation.endingpage | 104 | - |
dc.citation.publicationname | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.identifier.doi | 10.1007/s00253-013-5285-z | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Lee, Se Jin | - |
dc.contributor.nonIdAuthor | Kang, Kyoung-Hee | - |
dc.contributor.nonIdAuthor | Lee, Hyuk | - |
dc.contributor.nonIdAuthor | Oh, Young Hoon | - |
dc.contributor.nonIdAuthor | Lee, Seung Hwan | - |
dc.contributor.nonIdAuthor | Park, Si Jae | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Polyhydroxyalkanoate | - |
dc.subject.keywordAuthor | 2-ketobutyrate | - |
dc.subject.keywordAuthor | 2-ketobutyrate oxidase | - |
dc.subject.keywordAuthor | Propionyl-CoA synthetase | - |
dc.subject.keywordAuthor | Propionyl-CoA | - |
dc.subject.keywordAuthor | Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) | - |
dc.subject.keywordPlus | PROPIONATE-COA TRANSFERASE | - |
dc.subject.keywordPlus | UNRELATED CARBON-SOURCES | - |
dc.subject.keywordPlus | POLYLACTIC ACID | - |
dc.subject.keywordPlus | POLYHYDROXYALKANOATES | - |
dc.subject.keywordPlus | 3-HYDROXYVALERATE | - |
dc.subject.keywordPlus | SYNTHASE | - |
dc.subject.keywordPlus | 2-HYDROXYBUTYRATE | - |
dc.subject.keywordPlus | MICROORGANISMS | - |
dc.subject.keywordPlus | 1-PROPANOL | - |
dc.subject.keywordPlus | COPOLYMERS | - |
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