DC Field | Value | Language |
---|---|---|
dc.contributor.author | Song, Jae Yang | ko |
dc.contributor.author | Seo, Young Bin | ko |
dc.contributor.author | Hong, Soon-Kwang | ko |
dc.contributor.author | Chang, YongKeun | ko |
dc.date.accessioned | 2013-04-11T07:50:21Z | - |
dc.date.available | 2013-04-11T07:50:21Z | - |
dc.date.created | 2013-04-02 | - |
dc.date.created | 2013-04-02 | - |
dc.date.issued | 2013-02 | - |
dc.identifier.citation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.23, no.2, pp.149 - 155 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | http://hdl.handle.net/10203/173462 | - |
dc.description.abstract | Heterologous expression of a putative K+/H+ antiporter of Streptomyces coelicolor A3(2) (designated as sha4) in E. coli and Streptomyces hygroscopicus JCM4427 showed enhanced tolerance to K+ stress, acidic-pH shock, and/or geldanamycin production under K+ stress. In a series of K+ extrusion experiments with sha4-carrying E. coli deficient in the K+/H+ antiporter, a restoration of impaired K+ extrusion activity was observed. Based on this, it was concluded that sha4 was a true K+/H+ antiporter. In different sets of experiments, the sha4-carrying E. coil showed significantly improved tolerances to K+ stresses and acidic-pH shock, whereas sha4-carrying S. hygroscopicus showed an improvement in K+ stress tolerance only. The sha4-carrying S. hygroscopicus showed much higher geldanamycin productivity than the control under K+ stress condition. In another set of experiments with a production medium, the secretion of geldanamycin was also significantly enhanced by the expression of sha4. | - |
dc.language | English | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.subject | NA+/H+ ANTIPORTER | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | BACILLUS-SUBTILIS | - |
dc.subject | SALT-STRESS | - |
dc.subject | HELICOBACTER-PYLORI | - |
dc.subject | STREPTOMYCES | - |
dc.subject | OVEREXPRESSION | - |
dc.subject | ARABIDOPSIS | - |
dc.subject | H+ | - |
dc.subject | TRANSPORTERS | - |
dc.title | Heterologous Expression of a Putative K+/H+ Antiporter of S. coelicolor A3(2) Enhances K+, Acidic-pH Shock Tolerances, and Geldanamycin Secretion | - |
dc.type | Article | - |
dc.identifier.wosid | 000315547800003 | - |
dc.identifier.scopusid | 2-s2.0-84873823605 | - |
dc.type.rims | ART | - |
dc.citation.volume | 23 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 149 | - |
dc.citation.endingpage | 155 | - |
dc.citation.publicationname | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.identifier.doi | 10.4014/jmb.1207.07030 | - |
dc.contributor.localauthor | Chang, YongKeun | - |
dc.contributor.nonIdAuthor | Song, Jae Yang | - |
dc.contributor.nonIdAuthor | Seo, Young Bin | - |
dc.contributor.nonIdAuthor | Hong, Soon-Kwang | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | K+/H+ antiporter | - |
dc.subject.keywordAuthor | salt tolerance | - |
dc.subject.keywordAuthor | pH shock tolerance | - |
dc.subject.keywordAuthor | geldanamycin | - |
dc.subject.keywordPlus | NA+/H+ ANTIPORTER | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | BACILLUS-SUBTILIS | - |
dc.subject.keywordPlus | SALT-STRESS | - |
dc.subject.keywordPlus | HELICOBACTER-PYLORI | - |
dc.subject.keywordPlus | STREPTOMYCES | - |
dc.subject.keywordPlus | OVEREXPRESSION | - |
dc.subject.keywordPlus | ARABIDOPSIS | - |
dc.subject.keywordPlus | H+ | - |
dc.subject.keywordPlus | TRANSPORTERS | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.