Preparation of oligonucleotide arrays with high-density DNA deposition and high hybridization efficiency

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In DNA microarray produced by DNA-deposition technology, DNA-immobilization and -hybridization yields on a solid support are most important factors for its accuracy and sensitivity. We have developed a dendrimeric support using silylated aldehyde slides and polyamidoamine (PAMAM) dendrimers. An oligonucleotide array was prepared through a crosslinking between the dendrimeric support and an oligonucleotide. Both DNA-immobilization and -hybridization yields on the solid support increased by the modification with the dendrimers. The increase of the immobilization and hybridization efficiency seems to result from a three-dimensional arrangement of the attached oligonucleotide. Therefore, our dendrimeric support may provide a simple and efficient solution to the preparation of DNA microarrays with high-density DNA-deposition and high hybridization efficiency.
Publisher
KOREAN CHEMICAL SOC
Issue Date
2004-11
Language
English
Article Type
Article
Keywords

DENDRIMER MONOLAYERS; IMMOBILIZATION; MICROARRAYS; SUPPORTS; SURFACE; MICROCHIPS; ATTACHMENT; CHEMISTRY; SEQUENCE; PROBE

Citation

BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.25, no.11, pp.1667 - 1670

ISSN
0253-2964
URI
http://hdl.handle.net/10203/16226
Appears in Collection
CH-Journal Papers(저널논문)
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