DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, JW | ko |
dc.contributor.author | Jung, Yongwon | ko |
dc.contributor.author | Jung, YH | ko |
dc.contributor.author | Seo, JS | ko |
dc.contributor.author | Lee, Younghoon | ko |
dc.date.accessioned | 2010-01-08T05:43:12Z | - |
dc.date.available | 2010-01-08T05:43:12Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2004-11 | - |
dc.identifier.citation | BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.25, no.11, pp.1667 - 1670 | - |
dc.identifier.issn | 0253-2964 | - |
dc.identifier.uri | http://hdl.handle.net/10203/16226 | - |
dc.description.abstract | In DNA microarray produced by DNA-deposition technology, DNA-immobilization and -hybridization yields on a solid support are most important factors for its accuracy and sensitivity. We have developed a dendrimeric support using silylated aldehyde slides and polyamidoamine (PAMAM) dendrimers. An oligonucleotide array was prepared through a crosslinking between the dendrimeric support and an oligonucleotide. Both DNA-immobilization and -hybridization yields on the solid support increased by the modification with the dendrimers. The increase of the immobilization and hybridization efficiency seems to result from a three-dimensional arrangement of the attached oligonucleotide. Therefore, our dendrimeric support may provide a simple and efficient solution to the preparation of DNA microarrays with high-density DNA-deposition and high hybridization efficiency. | - |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | KOREAN CHEMICAL SOC | - |
dc.subject | DENDRIMER MONOLAYERS | - |
dc.subject | IMMOBILIZATION | - |
dc.subject | MICROARRAYS | - |
dc.subject | SUPPORTS | - |
dc.subject | SURFACE | - |
dc.subject | MICROCHIPS | - |
dc.subject | ATTACHMENT | - |
dc.subject | CHEMISTRY | - |
dc.subject | SEQUENCE | - |
dc.subject | PROBE | - |
dc.title | Preparation of oligonucleotide arrays with high-density DNA deposition and high hybridization efficiency | - |
dc.type | Article | - |
dc.identifier.wosid | 000225582700022 | - |
dc.identifier.scopusid | 2-s2.0-10644249889 | - |
dc.type.rims | ART | - |
dc.citation.volume | 25 | - |
dc.citation.issue | 11 | - |
dc.citation.beginningpage | 1667 | - |
dc.citation.endingpage | 1670 | - |
dc.citation.publicationname | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Jung, Yongwon | - |
dc.contributor.localauthor | Lee, Younghoon | - |
dc.contributor.nonIdAuthor | Park, JW | - |
dc.contributor.nonIdAuthor | Jung, YH | - |
dc.contributor.nonIdAuthor | Seo, JS | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | dendrimer | - |
dc.subject.keywordAuthor | DNA chip | - |
dc.subject.keywordAuthor | hybridization | - |
dc.subject.keywordAuthor | immobilization | - |
dc.subject.keywordAuthor | oligonucleotide | - |
dc.subject.keywordPlus | DENDRIMER MONOLAYERS | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | MICROARRAYS | - |
dc.subject.keywordPlus | SUPPORTS | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordPlus | MICROCHIPS | - |
dc.subject.keywordPlus | ATTACHMENT | - |
dc.subject.keywordPlus | CHEMISTRY | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | PROBE | - |
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