SUMO (small ubiquitin-like modifier) chip for analysis of SUMO-conjugation to a target protein

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The analysis of protein interactions and modifications in a microarray format has become the focus of great interest in researchers in drug discovery, diagnostics, and cell biology, due to the advent of rapid and high-throughput biological assays requiring only a small amount of sample. Herein, we describe a chip-based analysis of sumoylation, the post-translational modification (PTM) process involving the covalent attachment of the small ubiquitin-like modifier (SUMO) family protein to a target protein in a mammalian cell. The protein was expressed using an in vitro translation system and immobilized directly onto a glass slide using epitope tags fused to the protein. In this paper, the microarray-based analysis technique was shown to be a useful strategy for the identification of SUMO target proteins from preexisting protein pools and proteome arrays, in a high-throughput manner.
Publisher
Springer
Issue Date
2007-03
Language
English
Article Type
Article
Keywords

GTPASE-ACTIVATING PROTEIN; COMPLEX; MICROARRAYS; TECHNOLOGY; RANGAP1; UBC9

Citation

BIOCHIP JOURNAL, v.1, no.1, pp.28 - 34

ISSN
1976-0280
URI
http://hdl.handle.net/10203/14109
Appears in Collection
BS-Journal Papers(저널논문)
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