SUMO (small ubiquitin-like modifier) chip for analysis of SUMO-conjugation to a target protein

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dc.contributor.authorOh, Young-Heeko
dc.contributor.authorKim, Young-Pilko
dc.contributor.authorKim, Hak-Sungko
dc.date.accessioned2009-12-03T07:46:36Z-
dc.date.available2009-12-03T07:46:36Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2007-03-
dc.identifier.citationBIOCHIP JOURNAL, v.1, no.1, pp.28 - 34-
dc.identifier.issn1976-0280-
dc.identifier.urihttp://hdl.handle.net/10203/14109-
dc.description.abstractThe analysis of protein interactions and modifications in a microarray format has become the focus of great interest in researchers in drug discovery, diagnostics, and cell biology, due to the advent of rapid and high-throughput biological assays requiring only a small amount of sample. Herein, we describe a chip-based analysis of sumoylation, the post-translational modification (PTM) process involving the covalent attachment of the small ubiquitin-like modifier (SUMO) family protein to a target protein in a mammalian cell. The protein was expressed using an in vitro translation system and immobilized directly onto a glass slide using epitope tags fused to the protein. In this paper, the microarray-based analysis technique was shown to be a useful strategy for the identification of SUMO target proteins from preexisting protein pools and proteome arrays, in a high-throughput manner.-
dc.languageEnglish-
dc.language.isoen_USen
dc.publisherSpringer-
dc.subjectGTPASE-ACTIVATING PROTEIN-
dc.subjectCOMPLEX-
dc.subjectMICROARRAYS-
dc.subjectTECHNOLOGY-
dc.subjectRANGAP1-
dc.subjectUBC9-
dc.titleSUMO (small ubiquitin-like modifier) chip for analysis of SUMO-conjugation to a target protein-
dc.typeArticle-
dc.identifier.wosid000258632200003-
dc.type.rimsART-
dc.citation.volume1-
dc.citation.issue1-
dc.citation.beginningpage28-
dc.citation.endingpage34-
dc.citation.publicationnameBIOCHIP JOURNAL-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.contributor.localauthorKim, Hak-Sung-
dc.contributor.nonIdAuthorOh, Young-Hee-
dc.contributor.nonIdAuthorKim, Young-Pil-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorPTM (post translational modification)-
dc.subject.keywordAuthorSUMO (small ubiquitin-like modifier)-
dc.subject.keywordAuthorprotein microarray-
dc.subject.keywordAuthorcell-free expression system-
dc.subject.keywordPlusGTPASE-ACTIVATING PROTEIN-
dc.subject.keywordPlusCOMPLEX-
dc.subject.keywordPlusMICROARRAYS-
dc.subject.keywordPlusTECHNOLOGY-
dc.subject.keywordPlusRANGAP1-
dc.subject.keywordPlusUBC9-
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