Direct recovery of intracellular proteins from Candida utilis using reverse micelles in combination with reducing agent
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | HAN, DH | ko |
| dc.contributor.author | Lee, SangYup | ko |
| dc.contributor.author | HONG, WH | ko |
| dc.date.accessioned | 2009-11-20T06:05:58Z | - |
| dc.date.available | 2009-11-20T06:05:58Z | - |
| dc.date.created | 2012-02-06 | - |
| dc.date.created | 2012-02-06 | - |
| dc.date.created | 2012-02-06 | - |
| dc.date.created | 2012-02-06 | - |
| dc.date.created | 2012-02-06 | - |
| dc.date.issued | 1993-08 | - |
| dc.identifier.citation | BIOTECHNOLOGY TECHNIQUES, v.7, no.8, pp.545 - 550 | - |
| dc.identifier.issn | 0951-208X | - |
| dc.identifier.uri | http://hdl.handle.net/10203/13000 | - |
| dc.description.abstract | Intracellular proteins were directly extracted from Candida utilis using reverse micelles in combination with a reducing agent. Extraction at pH 10 using 80 mM cetyl trimethyl ammonium bromide (CTAB) and 0.2 % 2-mercaptoethanol was the most favorable condition for cell permeabilization and protein solubilization. Stripping at pH 7 in the presence of 0.5 M KCl resulted in the efficient recovery of solubilized proteins. | - |
| dc.language | English | - |
| dc.language.iso | en_US | en |
| dc.publisher | CHAPMAN HALL LTD | - |
| dc.title | Direct recovery of intracellular proteins from Candida utilis using reverse micelles in combination with reducing agent | - |
| dc.type | Article | - |
| dc.identifier.wosid | A1993LT10600001 | - |
| dc.identifier.scopusid | 2-s2.0-0010689271 | - |
| dc.type.rims | ART | - |
| dc.citation.volume | 7 | - |
| dc.citation.issue | 8 | - |
| dc.citation.beginningpage | 545 | - |
| dc.citation.endingpage | 550 | - |
| dc.citation.publicationname | BIOTECHNOLOGY TECHNIQUES | - |
| dc.embargo.liftdate | 9999-12-31 | - |
| dc.embargo.terms | 9999-12-31 | - |
| dc.contributor.localauthor | Lee, SangYup | - |
| dc.contributor.nonIdAuthor | HAN, DH | - |
| dc.contributor.nonIdAuthor | HONG, WH | - |
| dc.type.journalArticle | Article | - |
| dc.subject.keywordPlus | PURIFICATION | - |
| dc.subject.keywordPlus | EXTRACTION | - |
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