Regulation of Dyrk1A kinase activity by 14-3-3

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Dual-specificity tyrosine(Y) regulated kinase 1A (DYRK1A) is a serine/threonine protein kinase implicated in mental retardation resulting from Down syndrome. In this study, we carried out yeast two-hybrid screening to find proteins regulating DYRK1A kinase activity. We identified 14-3-3 as a Dyrk1A interacting protein, which is consistent with the previous finding of the interaction between the yeast orthologues Yak1p and Bmh1/2p. We showed the interaction between Dyrk1A and 14-3-3 in vitro and in vivo. The binding required the N-terminus of Dyrk1A and was independent of the Dyrk1A phosphorylation status. Functionally, 14-3-3 binding increased Dyrk1A kinase activity in a dose dependent manner in vitro. In vivo, a small peptide inhibiting 14-3-3 binding, sc138, decreased Dyrk1A kinase activity in COS7. In summary, these results suggest that DYRK1A kinase activity could be regulated by the interaction of 14-3-3.
Publisher
Elsevier
Issue Date
2004-10-15
Keywords

DYRK1A; 14-3-3; Kinase activity; Down syndrome

Citation

Biochemical and Biophysical Research Communications, Vol.323, No.2, pp.499-504

DOI
10.1016/j.bbrc.2004.08.102
URI
http://hdl.handle.net/10203/10710
Appears in Collection
BS-Conference Papers(학술회의논문)

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