DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Doyeun | - |
dc.contributor.author | Won, Jungyeon | - |
dc.contributor.author | Shin, Dong Wook | - |
dc.contributor.author | Kang, Junghee | - |
dc.contributor.author | Kim, Yeon Ju | - |
dc.contributor.author | Choi, Su Young | - |
dc.contributor.author | Hwang, Mi-Kyung | - |
dc.contributor.author | Jeong, Byeong-Woo | - |
dc.contributor.author | Kim, Gun Soo | - |
dc.contributor.author | Joe, Cheol O. | - |
dc.contributor.author | Chung, Sul-Hee | - |
dc.contributor.author | Song, Woo-Joo | - |
dc.date.accessioned | 2009-08-24T06:32:11Z | - |
dc.date.available | 2009-08-24T06:32:11Z | - |
dc.date.issued | 2004-10-15 | - |
dc.identifier.citation | Biochemical and Biophysical Research Communications, Vol.323, No.2, pp.499-504 | en |
dc.identifier.uri | http://hdl.handle.net/10203/10710 | - |
dc.description.abstract | Dual-specificity tyrosine(Y) regulated kinase 1A (DYRK1A) is a serine/threonine protein kinase implicated in mental retardation resulting from Down syndrome. In this study, we carried out yeast two-hybrid screening to find proteins regulating DYRK1A kinase activity. We identified 14-3-3 as a Dyrk1A interacting protein, which is consistent with the previous finding of the interaction between the yeast orthologues Yak1p and Bmh1/2p. We showed the interaction between Dyrk1A and 14-3-3 in vitro and in vivo. The binding required the N-terminus of Dyrk1A and was independent of the Dyrk1A phosphorylation status. Functionally, 14-3-3 binding increased Dyrk1A kinase activity in a dose dependent manner in vitro. In vivo, a small peptide inhibiting 14-3-3 binding, sc138, decreased Dyrk1A kinase activity in COS7. In summary, these results suggest that DYRK1A kinase activity could be regulated by the interaction of 14-3-3. | en |
dc.description.sponsorship | Division of CNS, Hanwha Chemical R&D Center, Daejon 305-345, Republic of Korea | en |
dc.language.iso | en_US | en |
dc.publisher | Elsevier | en |
dc.subject | DYRK1A | en |
dc.subject | 14-3-3 | en |
dc.subject | Kinase activity | en |
dc.subject | Down syndrome | en |
dc.title | Regulation of Dyrk1A kinase activity by 14-3-3 | en |
dc.type | Article | en |
dc.identifier.doi | 10.1016/j.bbrc.2004.08.102 | - |
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