Label-Free Electrochemical Diagnosis of Viral Antigens with Genetically Engineered Fusion Protein

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We have developed a simple electrochemical biosensing strategy for the label-free diagnosis of hepatitis B virus (HBV) on a gold electrode surface. Gold-binding polypeptide (GBP) fused with single-chain antibody (ScFv) against HBV surface antigen (HBsAg), in forms of genetically engineered protein, was utilized. This GBP-ScFv fusion protein can directly bind onto the gold substrate with the strong binding affinity between the GBP and the gold surface, while the recognition site orients toward the sample for target binding at the same time. Furthermore, this one-step immobilization strategy greatly simplifies a fabrication process without any chemical modification as well as maintaining activity of biological recognition elements. This system allows specific immobilization of proteins and sensitive detection of targets, which were verified by surface plasmon resonance analysis and successfully applied to electrochemical cyclic voltammetry and impedance spectroscopy upto 0.14 ng/mL HBsAg.
Publisher
MDPI AG
Issue Date
2012-08
Language
English
Article Type
Article
Citation

SENSORS, v.12, no.8, pp.10097 - 10108

ISSN
1424-8220
DOI
10.3390/s120810097
URI
http://hdl.handle.net/10203/103106
Appears in Collection
CBE-Journal Papers(저널논문)
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