DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hong, Chansik | ko |
dc.contributor.author | Kim, Jinsung | ko |
dc.contributor.author | Jeon, Jae-Pyo | ko |
dc.contributor.author | Wie, Jinhong | ko |
dc.contributor.author | Kwak, Misun | ko |
dc.contributor.author | Ha, Kotdaji | ko |
dc.contributor.author | Kim, Hana | ko |
dc.contributor.author | Myeong, Jongyun | ko |
dc.contributor.author | Kim, Sung Young | ko |
dc.contributor.author | Jeon, Ju-Hong | ko |
dc.contributor.author | So, Insuk | ko |
dc.date.accessioned | 2013-03-12T13:07:50Z | - |
dc.date.available | 2013-03-12T13:07:50Z | - |
dc.date.created | 2012-08-20 | - |
dc.date.created | 2012-08-20 | - |
dc.date.issued | 2012-04 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.421, no.1, pp.105 - 111 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/10203/102418 | - |
dc.description.abstract | Canonical transient receptor potential (TRPC) channels are Ca2+-permeable, non-selective cation channels those are widely expressed in mammalian cells. Various molecules have been found to regulate TRPC both in vivo and in vitro, but it is unclear how heterotrimeric G proteins transmit external stimuli to regulate the activity of TRPC5. Here, we demonstrated that TRPC5 was potentiated by the G alpha(s) regulatory pathway. Whole-cell TRPC5 current was significantly increased by beta-adrenergic receptor agonist, isoproterenol (ISO, 246 +/- 36%, n = 6), an activator of the adenylate cyclase, forskolin (FSK, 273 +/- 6%, n = 5), or a membrane permeable cAMP analogue, 8-Br-cAMP (251 +/- 63%, n = 7). In addition, robust Ca2+ transient induced by isoproterenol was observed utilizing a Ca2+ imaging technique. When intracellular [Ca2+](i) was buffered to 50 nM, cAMP-induced potentiation was attenuated. We also found that the Ca2+ release is mediated by IP3 since intracellular IP3 infusion attenuated the potentiation of TRPC5 by G alpha(s) cascade. Finally, we identified that the membrane localization of TRPC5 was significantly increased by ISO (155 +/- 17%, n = 3), FSK (172 +/- 39%, n = 3) or 8-Br-cAMP (216 +/- 59%, n = 3). In conclusion, these results suggest that the G alpha(s)-cAMP pathway potentiates the activity of TRPC5 via facilitating intracellular Ca2+ dynamics and increasing channel trafficking to the plasma membrane. (C) 2012 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | NONSELECTIVE CATION CHANNELS | - |
dc.subject | SIGNALING PATHWAY | - |
dc.subject | ACTIVATION | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | STIMULATION | - |
dc.subject | INSERTION | - |
dc.subject | CYCLASE | - |
dc.title | Gs cascade regulates canonical transient receptor potential 5 (TRPC5) through cAMP mediated intracellular Ca2+ release and ion channel trafficking | - |
dc.type | Article | - |
dc.identifier.wosid | 000304501400019 | - |
dc.identifier.scopusid | 2-s2.0-84860322598 | - |
dc.type.rims | ART | - |
dc.citation.volume | 421 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 105 | - |
dc.citation.endingpage | 111 | - |
dc.citation.publicationname | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.identifier.doi | 10.1016/j.bbrc.2012.03.123 | - |
dc.contributor.localauthor | Kim, Jinsung | - |
dc.contributor.nonIdAuthor | Hong, Chansik | - |
dc.contributor.nonIdAuthor | Jeon, Jae-Pyo | - |
dc.contributor.nonIdAuthor | Wie, Jinhong | - |
dc.contributor.nonIdAuthor | Kwak, Misun | - |
dc.contributor.nonIdAuthor | Ha, Kotdaji | - |
dc.contributor.nonIdAuthor | Kim, Hana | - |
dc.contributor.nonIdAuthor | Myeong, Jongyun | - |
dc.contributor.nonIdAuthor | Kim, Sung Young | - |
dc.contributor.nonIdAuthor | Jeon, Ju-Hong | - |
dc.contributor.nonIdAuthor | So, Insuk | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | TRPC | - |
dc.subject.keywordAuthor | Gs | - |
dc.subject.keywordAuthor | Beta-adrenergic receptor | - |
dc.subject.keywordAuthor | Isoproterenol | - |
dc.subject.keywordAuthor | cAMP | - |
dc.subject.keywordAuthor | Ca2+ | - |
dc.subject.keywordPlus | NONSELECTIVE CATION CHANNELS | - |
dc.subject.keywordPlus | SIGNALING PATHWAY | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | STIMULATION | - |
dc.subject.keywordPlus | INSERTION | - |
dc.subject.keywordPlus | CYCLASE | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.